The Glycine species are the important gene pools for improvement of soybean crop against insect, disease and drought stress, comparing to the narrower germplasm of the cutivar soybean. Identifying useful trait doners as parents for breeding effectively can facilitate the breeding program. Many experiments had confirmed that seed proteins electrophoresis can be used as a tool for plant taxonomy, and the efficiency will increase if collocate with Western blotting. In the present study, the seed proteins from several Glycine species were analyzed by sodium dodecyl sulfate-gel electrophoresis (SDS-PAGE) and Western blot against soybean seed maturation protein and seed storage protein antibodies. Each species yields a unique electrophoretic pattern that varied in the total number of bands and their relative mobilities. Thus, we may use these protein profiles as a tool to identify Glycine species. Six seed protein products, including MP130, Glycinin, GmPM4, GmPM5, GmPM16, and GmPM28, are less varied, and the other three proteins, GmPM1, GmPM2 and Gm PM8, are more varied among Glycine species. The results thus gave a similar conclusion as those provided by isozyme studies described previously by other group.