Motivation: The (superscript 99m)Tc-DMSA renal cortical scan is a regular examination in diagnostic acute pyelonephritis. It is non-invasive, safe care, and soothing relief for babies. However, it needs thirty minutes for (superscript 99m)Tc-DMSA renal cortical scanning. Static mode is difficult to image babies and young children without motion during thirty minutes scanning. Conventionally, (superscript 99m)Tc-DMSA is often required to image and process scanning while baby or child is asleep. Nevertheless, SPECT images are noisy during the rotation. Especially, the SPECT makes louder noise during rotation of views and often awake the sleeping child. That leads to examination failure or image displacement. In this study, the main aim is applied continuous mode in SPECT scanning protocol to eliminate the motion artifact and to provide smoothing examination for child. However, the image quality will be affected certainly under continuous mode. For this reason, we analyze and find scanning protocols and parameters without decreasing image quality under continuous mode. Materials and methods: The imaging scanner is Siemens E.CAM. Flanged Jaszczak ECT phantom was applied to investigate scanning protocols and conditions between continuous mode (CM) and step and shoot mode (SSM). There were three different acquisition times including 30, 37, and 45 seconds per view. 32 and 48 views per head were designed to evaluate the quality of images. The optimal scanning conditions and analysis of image quality were investigated and compared with cold spots of phantom images. Result: There is zero, 60%, 40%, and 100% significant between CM and SSM while time of acquisition and views of head were (30, 32), (30, 48), (45, 32), and (45, 48) with respectively to seconds and views. Conclusion: In this study, the optimal scanning conditions were 30 seconds of view and 32 views of head. It not only reduces louder noise, but also keeps quality of image between SSM and CM. Meanwhile, CM of SPECT imaging is feasible to apply baby or young child.