The research probes into how local actors participate in and construct the commodity chain from the dynamic development of global frozen tuna commodity chain from the year 1950 to 2000. Applying the concept of ‘mode of ordering’ in Actor Network Theory, I try to comprehend how different actors organize natural, social, economic and cultural heterogeneous elements, shaping the relational effect of the change of global commodity chain. Under the restructuring and globalization of agro-food system, the agricultural issue derives different interpretations in the field of economic geography. Affected by ‘cultural turn’ in economic geography, culture, nature and social relations are rediscovered, so that more researchers concern the cultural, social embeddedness and dynamic forces of actors in the agro-food study. When the global frozen tuna commodity chain turned to global outsourcing, actors in Taiwan effectively apply practices of low cost and fast development to change the commodity chain. After the year 2000, under the pressure of decreasing resources, because the tuna resource regulation organizations and Japan enforce the regulation measures, Taiwan actors try to evade regulations and to transform into vertically integrated corporations. The turn from ‘space of prescription’ to ‘space of negotiation’ of the mode of ordering revels how the different actor networks mutually construct the dynamic change of commodity chain.