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Random Amplified DNA Disclosed Genomic Instability in Successive Cutting-Propagated Clones of Sweet Potato (Ipomoea Batatas (L) Lam.) with N-Fertilizer Treatments

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Random amplified polymorphic DNA was used on two sweet potato cultivars, TN57 and TN66, for detecting genomic variations among tissues of different developmental stages and in plants across successive cutting generations grown under different cycles of nitrogen fertilizer (N-fertilizer) treatments. From total of 35 random primers screened, 15 primers were found to be informative for detecting differences either between varieties or among shoot sprout, primary leaf and mature leaf DNAs. Among the 15 primers, it was found that 8 primers showed a consistency in amplified band patterns in each variety regardless of tissue types while the rest of 7 primers indicated polymorphisms among tissues. Six of the later 7 primers were further used for the studies of genetic stability in successive cutting propagated generations and N-fertilizer treatments. All the six primers were capable of detecting differences among clones derived from different cutting generations and/or cutting clones grown under regular- and extra-N-fertilizer treatments. Genomic instability revealed in N-fertilizer treatments and cutting generation differences were found to be variety-dependent. The observed differences probed by random primers suggested that some proportion of plant genome might tend to readjust and subject to change during the courses of intra-plant development, different growth periods and/or under different cultural conditions(N-nutrient treatments in our case).

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