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Antioxidant and Antimicrobial Activities of Ophiocordyceps sinensis Cultivated in Adlay-based Media


Ophiocordyceps sinensis is a fungus that has been used in traditional Chinese medicine for at least 2000 years. This study investigated the antioxidant and antimicrobial activities of the mycelial extracts of O. sinensis cultivated in adlay-based media. Mycelia of two wild strains of O. sinensis cultivated in adlay broth media had a polysaccharide concentration of 22.8-25.4 mg/ mL and produced a low concentration of cordycepin (0.4 mg/mL). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and Trolox equivalent antioxidant capacity (TEAC) of mycelial extracts were 71%-79% and 0.5-0.7 mM, respectively. The mycelial extracts of O. sinensis cultivated in the adlay solid substrate media produced a significantly higher amount of polysaccharide (736.6-778.3 mg/mL) and cordycepin (1.7-1.8 mg/mL). When O. sinensis was cultivated in adlay-based media, the DPPH radical scavenging activity (81%-90%) and TEAC (0.7-0.8 mM) increased significantly. Antimicrobial activity assays of the fermentation broth indicated that the two wild strains of O. sinensis exhibited inhibitory activities against grampositive bacteria, such as Staphylococcus aureus, and gram-negative bacteria, such as Escherichia coli and Pseudomonas aeruginosa.