薑精油對肝癌細胞及正常肝細胞生理機能之影響

Effect of ginger essential oil on the physiological functions of hepatoma cells and normal hepatocytes

陳嘉琳(Chia-Lin Chen)

指導教授 ： 沈立言

共同指導教授 ： 張鴻民

國立臺灣大學/生物資源暨農學院/食品科技研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.00694

摘要

本研究採用南投縣所栽培之生薑（Zingiber officinale Roscoe），以水蒸氣蒸餾法所萃取之薑精油為實驗材料，以 MTT assay 來探討不同濃度的薑精油對於人類肝癌細胞株Hep G2與Hep 3B之抑制率。並採用大白鼠初代肝細胞之分離與培養為實驗模式，探討不同濃度之薑精油對大白鼠初代肝細胞生存力、抗氧化及解毒代謝能力之影響。實驗結果顯示，薑精油平均萃取率為0.16％。經由GC-MS分析，精油成份含有高量的萜烯類碳氫化合物，尤以香葉醛(geranial)含量高達25.2％，香茅醇(citronellol)次之，約為18.7％。對於肝癌細胞株Hep G2而言，低濃度10 μg/ml薑精油處理24小時後無顯著性抑制效果，而50~200 μg/ml薑精油處理下則能顯著性抑制肝癌細胞Hep G2之增生。IC50為93.3 μg/ml。而高濃度100~200 μg/ml薑精油處理肝癌細胞Hep 3B 24小時後細胞生存力抑制率皆顯著高於控制組(p＜0.05)，IC50為185.7 μg/ml。可見薑精油對於肝癌細胞Hep G2之抑制效果較Hep 3B好。在薑精油低濃度（50 μg/ml）情況下，對於肝癌細胞Hep G2有28.1％生長抑制效果，但對正常大白鼠初代肝細胞則無細胞毒性。而當薑精油濃度0-100 μg/ml時，對於正常大白鼠初代肝細胞的TBARS值無影響，也就表示0-100 μg/ml的薑精油不會誘發正常大白鼠初代肝細胞的脂質過氧化。另一方面，2.5 μg/ml的薑精油則能顯著增加正常初代肝細胞的麩胱甘肽(glutathione, GSH)含量，並且提升抗氧化及解毒代謝系統的麩胱甘肽過氧化酶(glutathione peroxidase) 以及麩胱甘肽還原酶(glutathione reductase)的活性。

關鍵字

細胞生存力 ； 薑 ； 肝癌

並列摘要

Abstract
The objectives of this study were to investigate the effects of various concentrations of ginger essential oil on cell viability, antioxidation and detoxification system in hepatoma cells, Hep G2 and Hep 3B, and primary rat hepatocytes. Ginger essential oil (GEO) was extracted from Zingiber officinale Roscoe by steam distillation. The cell viability of hepatoma cell lines Hep G2 and Hep 3B, which were treated with various concentrations of GEO, was investigated by MTT assay. In addition, the cell viability, antioxidation and detoxification systems of primary rat hepatocytes treated with various concentrations of GEO were also investigated. Results showed that the average yield of extraction of fresh ginger was 0.16%, the major components of the GEO were geranial (25.2 %) and citronellol (18.7 %) in GEO based on the GC-MS analysis. In MTT assay, when the Hep 3B cells were treated with high concentrations of GEO (100~200 μg/ml) for 24 hours, the inhibition percentages were significantly higher than that of the control (p＜0.05). The IC50 of Hep 3B was around 173 μg/ml of GEO. For Hep G2 treated with 50~200 μg/ml of GEO, inhibition percentages of cell viability were also significantly higher than that of the control (p＜0.05). The IC50 of Hep G2 was about 94.2 μg/ml of GEO. These results supgested that the inhibition effect of GEO on Hep G2 was much better than on Hep 3B. Under low concentration of GEO (50

並列關鍵字

cell viability ； ginger ； liver cancer

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