- 學位論文
應用雙光子顯微術觀測氧化鋅奈米粒子於化學增益下之皮膚穿透深度
Utilizing Multiphoton Microscopy to Investigate Chemically Enhanced Transdermal Delivery Depth of ZnO NPs
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摘要
雙光子顯微術由於使用波長較長的近紅外光作為激發光源，所以具有穿透深度較深、較少的光破壞，且可以激發所有可見光波段的自發螢光訊號和二倍頻訊號，使得我們可以直接透過自發螢光訊號和二倍頻訊號來觀測樣品。因此，我們希望能將這種非線性光學顯微術技術應用於皮膚上的觀測與分析。 本篇文章目的是利用雙光子顯微術及化學增益法去觀測氧化鋅奈米粒子滲透皮膚之深度以及在角質層的分佈情形。首先我們使用的是裸鼠的鼠皮，主要是避免鼠毛自發螢光的幹擾，然後我們將酒精、油酸和PBS配置成四種不同的溶劑，分別加入氧化鋅奈米粒子，再將其放於裸鼠皮上泡皮12小時。利用雙光子顯微術所擷取的裸鼠皮自發螢光訊號以及氧化鋅奈米粒子的二倍頻訊號，能夠用來觀察氧化鋅奈米粒子在角質層的分佈情形與化學增益劑下的滲透深度。由於化學性增益劑的使用，誘使改變氧化鋅奈米粒子的一些增益係數，而這些係數可以由實驗數據加以量化。 實驗結果指出氧化鋅奈米粒子主要分佈在角質層的細胞間隙，因此主要是藉由細胞間隙進行經皮吸收，並無觀察到氧化鋅直接穿透角質細胞的現象，而化學增益下的氧化鋅奈米粒子的穿透深度並未有明顯增加，其仍然停留在表皮層，並未深入真皮層。
並列摘要
Multiphoton microscopy , using longer wavelength of near infrared light as excitation light source has deeper penetration and less photodamage.Besides , it can capable all visible autofluorescence and second harmonic generation , making us observe specimens through autofluorescence and second harmonic generation directly without slicing and dying . Hence , I hope to apply this technology of nonlinear optical microscopy to observe and analysize in skin . The aim of this research is to utilizing multiphoton microscopy to investigate chemically enhanced transdermal delivery depth of ZnO NPs and the distribution of ZnO NPs in the stratum corneum . First , we chose the nude mice skin to avoid the autofluorescence of hair . The skin was incubated in the diffusion cell with four diffusion solution for 12 hours . Then we used the data to investigate the penetration depth and the distribution of ZnO NPs . We also can quantify the transport properties of the ZnO NPs under chemical enhancers . In this work, the results demonstrate that ZnO NPs mainly distribute in the stratum corneum . The chemical enhancements didn’t increase the penetration depth obviously. ZnO NPs penetrate beyond SC, not into the dermis.
參考文獻
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