- 學位論文
含銀膠原蛋白敷材之抗菌與癒傷效能研究
Study for antimicrobial and wound healing efficacy of silver-collagen dressing
摘要
皮膚是人體面積最廣的器官之一，負責重要的屏蔽功能，是阻絕外來病原入侵的第一道防線。一旦皮膚完整性因傷口而被破壞時，屏蔽功能受損，就可能產生感染的問題。感染會使傷口長期處於發炎期，造成傷口正常癒合功能受損，嚴重時會引發組織壞死導致截肢，甚至引發敗血症危害病患生命。因此，如何同時達成有效控制感染並促進傷口癒合是當前傷口護理的一大重要議題。 現有市面上的醫療敷材多數只個別講求抗菌或為只能單一促進傷口癒合的敷材，為了解決這項缺失，本研究結合膠原蛋白在促進傷口癒合上之優勢與銀化合物的抗菌效能，藉由膠原蛋白作為銀化合物釋放系統，開發出『促進傷口癒合』與『穩定抗菌』之抗菌敷材；同時，本研究也建立一系列體外抗菌能力測試與動物感染性傷口模式，以分析該抗菌敷材之抗菌與促進傷口癒合效能。本研究結果顯示：在in vitro抗菌測試中，含銀膠原蛋白綿的在24小時對Escherichia coli抑菌百分比為99.9961%，對Pseudomonas aeruginosa的抑菌百分比為99.7172%（對應control group菌數），在in vivo中能有效將菌數抑制在105 cfu/g 以下，並促使傷口快速癒合。
並列摘要
Skin, one of the largest organs in human body, covers whole body surface and functions as a first line of defense mechanisms against the invasion of foreign microbes. Once the integrity of skin is disrupted by an open wound, the protective barrier is lost and the wounded area could provide an entry site for the foreign microorganisms. Microbial infection will perturb and delay the progression of normal wound healing and causes the chronicity of cutaneous wound. Severe infection could cause extensive tissue necrosis, amputation, systemic sepsis, and death. In general, most of commercial wound dressings deal with either wound infection or wound healing. To control infection and improve wound healing simultaneously, we constructed a dressing combined with collagen and silver compounds. Collagen is a major component of connective tissues and plays an important role in normal wound healing, especially in the granulation phase. The advantages of collagen include high biocompatibility, non-cytotoxicity, low immunogenicity, biodegradability, and bioresorption. Collagen is also attractive to be a flexible drug delivery system. The silver compounds have powerfully antimicrobial activity against a broad spectrum of microbes, including common wound pathogens. We also establish the in vitro antimicrobial tests and an in vivo infected wound model to assay the antimicrobial efficacy of this dressing. In summary, we demonstrated the dressing comprised of both collagen and silver compounds not only effectively controlled the infection but also significantly promoted the wound healing.