摻銀類鑽碳奈米複合薄膜之生物植入和抗菌性質

Bio-implantation and Anti-bacterial properties of the Ag-doped DLC nanocomposite films

林明宏(Ming-Hong Lin)

指導教授 ： 林啟瑞

共同指導教授 ： 李仁愛(Jen-Ai Lee)

國立臺北科技大學/機電學院/機電整合研究所/碩士(2014年)

https://doi.org/10.6841/NTUT.2014.00242

摘要

類鑽碳薄膜擁有許多良好的物理性質與化學性質，其生物相容性更是增加了類鑽碳在生醫材料之應用。本論文之研究使用R.F非平衡磁控濺鍍系統沉積類鑽碳薄膜，探討不同工作壓力參數對於薄膜的差異，接著討論其物理與化學性質之變化；之後藉由系統內之雙濺鍍槍進行摻銀類鑽碳奈米複合薄膜之沉積，可以發現隨著銀元素共鍍時間的不同，薄膜表面形成不同大小的奈米銀粒子且表面粗糙度與潤濕角有所上升之情況。
在生物實驗上，研究中分別進行細胞與細菌之體外培養，實驗選用MG-63細胞與大腸桿菌個別培養於不同基材上，並觀察其細胞與細菌的貼附與生長情況。實驗研究首先觀察MG-63細胞培養一天之貼附情況，可發現在摻銀類鑽碳奈米複合薄膜上並沒有細胞貼附在上面；但在細胞存活率分析中，其細胞存活率高於其餘基板與薄膜。大腸桿菌的實驗中，純類鑽碳薄膜上的細菌貼附情況少於矽基板與生醫金屬，其可能代表薄膜之性質不適合細菌之貼附；而摻銀類鑽碳奈米複合薄膜表面幾乎沒有任何的大腸桿菌殘留，證實了含銀類鑽碳複合薄膜的確擁有其抗菌性。

關鍵字

類鑽碳薄膜 ； 摻銀類鑽碳奈米複合薄膜 ； 生物相容性 ； 抗菌性

並列摘要

Diamond-like carbon(DLC) films have a lot of physical and chemical properties, like high hardness, electrical resistance, chemical inertness, especially ensure high biocompatibility. In this study, DLC and Ag-doped DLC(Ag-DLC) nanocoposite films were deposited on silicon substrate by R.F. unbalanced magnetron sputtering technique. It is shown that the DLC films had smooth surface and good chemical inertness. Ag-DLC nanocoposite films had different nano-silver sizes, higher hydrophobicity and surface roughness.
In biological experiments, thin films were conducted in cell culture and bacteria experiment. MG-63 cells were cultured on substrates of Si、Ti6Al4V、glass、DLC and Ag-doped DLC nanocoposite films. The vitro tests revealed that MG-63 cell attached on DLC films but Ag-doped DLC nanocoposite films not and cytotoxicity tests shown Ag-doped DLC nanocoposite films did not release toxicity. In anti-bacterial test, DLC films had little number of E. coli attached on surface. Ag-doped DLC nanocoposite films surface did not have any E. coli attached and killed E.coli in bacterial solution. It confirmed Ag-doped DLC nanocoposite films have good antibacterial properties.

並列關鍵字

Diamond-like carbon thin films ； Ag-doped DLC thin films ； Biocompatibility ； Anti-bacterial

參考文獻

[9] 蔡潔娃，生醫材料之發展與應用，金屬中心，2010。

[61] 謝榮騰，氫化及鈦摻雜類鑽碳膜之生物相容性和抗菌性，碩士論文，國立台北科技大學，2013年。

[3] C. Hinuber, C. Kleemann, R. J. Friederichs, L. Haubold, H. J. Scheibe1, T. Schuelke, C. Boehlert and M. J. Baumann, “Biocompatibility and mechanical properties of diamond-like coatings on cobalt-chromium-molybdenum steel and titanium-aluminum-vanadium biomedical alloys,” Journal of Biomedical Materials Research Part A, Volume 95A, Issue 2, pages 388-400, November 2010.

[4] FR Marciano, LF Bonetti, LV Santos, NS Da-Silva, EJ Corat and VJ Trava-Airoldi, “Antibacterial activity of DLC and Ag-DLC films produced by PECVD technique, ” Diamond and Related Materials, Volume 18, Issues 5-8, May-August 2009, Pages 1010-1014.

[5] ISO 10993-1:1992 Biological evaluation of medical devices: Part 1: Evaluation and testing.