- 學位論文
以快速原型沉積法製作3D多孔纖維材質皮膚敷料
Preparation of 3D Fibrous Wound Dressings With Rapid Prototyping Technique
摘要
理想情況下，皮膚傷口敷料可排除傷口滲出物使傷口保持乾燥，提供濕潤的環境避免傷口脫水，讓氧氣流通，以幫助組織再生。傳統的水凝膠敷料，由於表面缺乏孔洞，氣體流通和清除滲出物能力欠佳。本研究以快速原型技術及濕紡技術，製造出褐藻酸鈉多孔性纖維材質皮膚敷料，並探討其物理化學性質與無孔洞薄膜作比較，評估是否適合作為皮膚傷口敷材。快速原型（RP）製作可控制纖維和孔洞大小的三維（3D）結構。我們也調查操作參數與敷材特性間的相關變化。這些參數包括出膠壓力、針頭孔徑、針頭移動速度、藻酸濃度和鈣離子濃度，以及針頭提高高度。褐藻酸水凝膠因其高生物相容性及生物降解性常被利用於組織工程和創傷敷料之應用。褐藻酸容易與鈣離子產生交聯(cross-link)，形成膠體。 以快速原型技術，在不同參數下製造出多孔性纖維材質皮膚敷料的物理化學性質結果顯示出增加出膠壓力、加大針頭孔徑和針頭提高高度，以及減少藻酸濃度、鈣離子濃度和針頭移動速度，均能使樣品纖維變粗且孔隙變小，此改變能有效降低樣品釋放鹽酸四環素效率、膨潤率、降解率，且增加樣品拉伸強度。透濕性測試結果顯示以快速原型技術製造之敷材透濕值略高於正常皮膚並低於無蓋容器(open cup)的透濕值，可有效保持傷口濕潤排除受傷後傷口滲出之水分。抗菌試驗結果顯示敷材緩慢釋放能抑制大腸桿菌的生長。與快速原型同樣參數下製出的濕紡樣品，孔隙相對較大且結構較為鬆散，導致濕紡樣品膨潤率、藥物釋放速率比快速原型樣品快，此外，濕紡樣品的拉伸強度較低。多孔纖維狀敷材與藻酸薄膜比較結果，薄膜具有較高的釋放率、膨潤率，進而提升楊氏係數。薄膜透濕性測試結果顯示會隨著褐藻酸濃度提高而下降，透濕性不佳。本實驗結果顯示快速原型沉積系統相較於薄膜與濕紡系統有較緩慢的藥物釋放效果能抑制大腸桿菌的生長以及較高的機械性質。
並列摘要
Ideally, skin wound dressings should keep the wound dry by allowing the evaporation of wound exudates and provide adequate moisture while avoiding the dehydration of the wound. They should also allow oxygen exchange to aid tissue regenerations. Traditional hydrogel slabs have the disadvantage of poor air circulation and little exudate removal capability due to the lack of pores in their structure. In this study, rapid prototyping and wet spinning technology to produce sodium alginate porous fibrous wound dressings, and to explore the physical and chemical properties compared with the non-porous film to assess the suitability of a skin wound dressings. The parameters included the pneumatic pressure, needle aperture, needle speed, alginate and calcium concentrations, and the distance between the needle tip and the top layer. Alginate is a biodegradable biocompatible natural polymer that has been used in tissue engineering applications. Alginate forms hydrogel when calcium ions are added in its polymer network. Results show that the increase in pneumatic pressure, needle aperture, needle distance and reduction in alginate, calcium ion concentrations and needle speed were able to increase the fiber diameters and reduce the porosities of the samples. Larger fibers and lower porosities reduced the samples’ release efficacies of tetracycline HCl (TCH), swelling ratios, degradation rates, and increase their tensile strengths. The results from the water vapor transmission test showed that all samples had higher water vapor transmission rates (WVTR) than the normal skin and much lower WVTR than an open cup. They were expected to better keep the skin wound moisturized. The antibacterial test showed the samples slowly release TCH could inhibit localized E. coli. growth. When the wet spinning sample is made under the same parameter as the rapid prototype, its pores are contrarily lager and its structure is much looser. This makes its swelling rate and the speed of drug release rate faster than rapid prototyping. In addition, the tensile strength of the wet spinning sample is lower. The results from the porous fibrous wound dressings are also compared to those of alginate film. Film has a high release rate, swelling rate, and thus enhance the Young's modulus. Film water vapor permeability test results show that the increase in alginate concentrations, moisture permeability is not good. The experimental results show that the rapid prototyping tooling system has slower drug release effects to be able to inhibit the growth of E. coli and higher mechanical properties than the wet-spinning system.