中草藥結合明膠應用於敷材之研究

Study of chinese herbal medicine dressings combined with gelatin

徐敏華(Min-Hua Hsu)

指導教授 ： 翁清松

中原大學/工學院/生物醫學工程研究所/碩士(2014年)

https://doi.org/10.6840/cycu201400844

摘要

當皮膚受到外來損傷而失去保護屏障之功能，則暴露之患處即有發炎與感染的風險，故對於傷口之管理顯得格外重要。爰此，本研究利用中國醫藥典籍記載之具有補血止血功效的中草藥阿膠及生物可降解性材料明膠為創傷敷材之主要基材材料，搭配三黃(大黃、黃芩、黃連)與石膏，以天然藥物取代化學合成品製備成創面修復敷材，應用於創傷敷材之研究。
實驗首先利用FT-IR與DSC測定，了解混合材料之物、化性質，再進行田口設計法，訂定實驗材料混合比例參數製作敷材成品，並分別討論五種不同的材料濃度(10%，15%，20%，25%，30%)以及三種不同的阿膠與明膠比例(70:30，50:50，30:70)，以拉伸、透氣度、接觸角等試驗測試，篩選出材料濃度暨比例之最佳參數，作為後續實驗依據；再以其包覆三黃萃取液與石膏，透過包覆率、釋放率、膨潤率實驗，了解本研究敷材之藥物載體特性。最後進行細胞毒性試驗，探討於敷材加入阿膠、三黃與石膏等中草藥後，是否擁有良好之物、化特性及生物相容性，並提供合適的癒合環境與加速創面之修復。
研究結果發現，於阿膠中添加明膠、三黃萃取液及石膏並與純阿膠組及純明膠組作比對，在FT-IR測定結果，吸收波長於各組樣本峰值無明顯變化，即加入其他材料不會影響阿膠與明膠的化學結構；而經DSC得知，加入不同藥材會使材料玻璃轉換溫度均落在材料穩定性範圍。透過成型實驗結果可知，濃度15%~25%才能製備出完整敷材；拉伸試驗結果顯示，各種材料比例均能提供敷材延展性，其中以濃度20%拉伸距離為最高值75.82 mm，且明膠比例與拉伸距離成正相關；透氣度試驗中，以濃度25%擁有較佳的透氣度，且明膠含量與其成負相關。透過上述最佳化參數實驗後，本研究遴選材料濃度20%及25%且阿膠暨明膠比例為50:50進行後續實驗。接觸角試驗中，以濃度20%擁有較為親水之性質，其角度介於60.44°~91.82°之間。包覆率實驗中，以濃度25%擁有90.38 %之包覆率為最高；釋放率實驗則以濃度20%擁有3.84 %之釋放率為最佳；膨潤率試驗中，兩實驗組皆有近原體積6倍之表現。由SEM觀察結果得知，濃度20%擁有5μm~20μm大小之孔洞；而MTT實驗結果顯示，材料濃度20%及25%之創傷敷材皆可使細胞顯著增生，證明其具有良好的生物相容性。
綜合上述實驗結果，所製備之創傷敷材具備成為臨床傷口敷材之良好特性及潛力，其中又以材料濃度20 %的實驗組擁有較大效益之優點，故於傷口癒合敷材的角色上，本研究將其選為最佳應用濃度，而在未來作為臨床創傷敷材的實際應用上，可針對敷材性質提升及動物/臨床試驗效果，做更深入的研究探討。

關鍵字

創傷敷材 ； 阿膠 ； 三黃 ； 明膠

並列摘要

When the skin loses its function to protect by external damage, the exposed surface is at risk of inflammation and infection, therefore the management of wounds is of great importance. This study utilized Chinese medicine Colla Corii Asini which was recorded in the classics of herbal medicine with hemostatic efficacy and a biodegradable material called gelatin, as the basic material of wound dressing. The wound dressing also used the natural medicine of San-Huang (Rhei Rhizoma, Scutellariae Radix, Coptidis Rhizoma) and gypsum to replace synthetic chemicals to prepare the dressing for wound healing and applications in research.
First, we used the FT-IR and DSC to test the physicochemical properties of mixed material. Then we determined the parameters of blending ratio of the material to produce the wound dressing by Taguchi Methods, and discussed the five different concentration of material (10%，15%，20%，25%，30%), three different blending ratio of Colla Corii Asini with gelatin (70:30，50:50，30:70), respectively. We utilized the experiment of the tensile test, water vapor transmission rate test and contact angle test to choose the best parameter of concentration and blending ratio of material, and used the best parameter to the other experiments which are in progress. Then, we used the wound dressing to encase the San-Huang and gypsum, and through the drug encapsulation efficiency, drug release efficiency and degree of swelling test in order to understand the properties of the drug carrier of wound dressing. Lastly, we studied the cytotoxicity assay and discussed whether the wound dressing had the physicochemical properties and biocompatibility. It is hoped that the wound dressing could provide a suitable environment to accelerate the wound healing.
The group adding gelatin, San-Huang extract and plaster in Colla Corii Asini was compared to the group with pure Colla Corii Asini as well as the group with gelatin. In the FT-IR results, no significant change in the absorption peak wavelength of samples in each group. By adding other material, the chemical structure of Colla Corii Asini and gelatin is not affected. Through DSC, adding different herbs can cause a transition in the glass material temperatures that is not in the range of material stability. For molding experimental results, a concentration of 15% to 25% is needed in order to prepare a complete cladding material. Tensile test results showed that the deposited materials can provide ductility in various proportions, in which the concentration of 20% has the highest value of stretching distance (75.82 mm). Experiments also showed that the ratio of gelatin with the stretching distance is positively correlated. For air permeability, the concentration of 25% has better air permeability which is proved by the negative correlation of the gelatin content. Through the above parameter optimization experiments, the materials that were used had a concentration of 20% and 25% for gelatin and gelatin-cum, with a ratio of 50:50 for subsequent experiments. For contact angle test, a concentration of 20% showed a more hydrophilic nature of the angle between 60.44 ° ~ 91.82 °. For coating rate, the concentration of 25% has the highest rate of 90.38% of the cladding. Release rate of 20% concentration experiment places owned 3.84% of the release rate of the best. Swelling ratio test, two experimental groups individually had nearly six times the performance of the original volume. SEM results showed that, the concentration of 20% has 5μm ~ 20μm size of the holes. The MTT results showed that the material concentration of 20% and 25% of the wound dressing material can be a significant proliferation of the cells, which proves it has a good biocompatibility.
Taking these experimental results, the materials have good characteristics and potential to become a clinical wound dressing material. Among them, the material having a concentration of 20% of the experimental group has the advantage of greater efficiency. Therefore the role in wound healing of the dressing material is selected as the best application of its concentration. And this dressing material as a practical application of clinical wound dressing materials, we can enhance the property of material and animal/clinical trial in-depth study in the future.

並列關鍵字

Colla Corii Asini ； gelatin ； San-Huang ； wound dressing

參考文獻

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