Have library access?
IP:3.236.116.27
  • Journals
  • OpenAccess

Effects of Crude Adlay Hull Acetone Extract on Steroidogenesis from Rat Zona Fasciculata-Reticularis Cells

薏苡殼丙酮粗萃取物對大鼠束網狀細胞類固醇生合成的效應

Abstracts


在中國薏苡仁用於醫療及食物,它俱有抑制突變及降血脂的功能。本文主要是探討用丙酮萃取薏苡仁殼,其對大鼠腎上腺皮質束網狀細胞類固醇生合成的影響。由實驗發現薏苡仁殼萃取液,抑制大鼠腎上腺皮質束網狀細胞皮質脂酮的分泌。透過動力學分析,發現薏苡仁殼萃取液分別透過不競爭性壓抑和非競爭性壓抑,影響腎上腺皮質束網狀細胞類固醇生合成的第一及最後酵素的活性,藉以抑制皮質脂酮的分泌。

Parallel abstracts


Adlay is a grass crop which has been used in traditional Chinese medicine and also as a nourishing food. It has been shown to posses anti-allergic, antimutagenic and hypolipemic effects. However, the steroidogenic effects of crude adlay hull acetone extract (AHA) on adrenal zona fasciculata-reticularis (ZFR) cells are still unclear. ZFR cells were incubated with AHA in the presence or absence of 25-hydroxy cholesterol (25-OH-cholesterol), pregnenolone, progesterone or deoxycorticosterone. The concentrations of corticosterone or pregnenolone in the media were measured by radioimmunoassay (RIA). The present data demonstrated that (1) AHA (800μg/ml) caused more pregnenolone release in control group, but not in 25-OH-cholesterol, trilostane or 25-OH-cholesterol+trilostane group; (2) kinetic study showed an uncompetitive inhibition model of AHA to P450 side chain cleavage enzyme (P450scc); (3) kinetic study showed a noncompetitive inhibition model of AHA to 11β-hydroxylase.

Cited by


鍾成沛(2011)。薏仁麩皮乙醇萃取物之活性篩選及其乙酸乙酯區分層之化學組成研究〔博士論文,國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2011.10497

Read-around