Phenotypic and Functional Analysis of Natural Killer Cells in Systemic Lupus Erythematosus Patients

全身性紅斑狼瘡患者自然殺手細胞表面標記及功能分析

江伯倫(Bor-Luen Chiang) ； 陳彥蓁(Yen-Chen Chen) ； 王崇任(Chrong-Reen Wang) ； 林玉玲(Yu-Ling Lin) ； 周正成(Chen-Cheng Chou) ； 謝貴雄(Kue-Hsiung Hsieh)

《Acta Paediatrica Sinica》 36卷5期 (1995/10) Pp. 357-363

https://doi.org/10.7097/APS.199510.0357

摘要

Systemic lupus erythematosus (SLE) manifested with multiple autoantibodies production and glomerulonephritis, is the best example of systemic autoimmune diseases. To further elucidate the role of cytokines and the potential involvement of natural killer cells (NK cells) in the pathogenesis of lupus, phenotypic analysis of peripheral blood mononuclear cells (PBMC), NK cells cytotoxicity and cytokines production pattern of SLE patients and normal controls were examined. In addition, the effect of a variety of cytokines on anti-dsDNA antibodies production was also investigated. Our results showed that: (a) there was an increased percentage of memory T cells and decreased percentage of NK cells in SLE patients when compared to normal controls (p＜0.05); (b) a decreased production of cytokines like γ-IFN in mitogen-stimulated PBMCs was also noted in SLE patients; (c) cytolytic activity of NK cells was markedly reduced in SLE patients (p＜0.05); (d) spontaneous secretion of IgG anti-dsDNA antibodies by B cells isolated from SLE patients could be inhibited by γ-IFN, but not by IL-2, IL-4 and IL-S. These data suggested that decreased functions of NK cells and related type I T helper cells be closely related to the immune dysregulation and autoantibodies production in SLE.

關鍵字

全身性紅斑狼瘡 ； 自然殺手細胞 ； T輔助細胞 ； 淋巴激素

並列摘要

Systemic lupus erythematosus (SLE) manifested with multiple autoantibodies production and glomerulonephritis, is the best example of systemic autoimmune diseases. To further elucidate the role of cytokines and the potential involvement of natural killer cells (NK cells) in the pathogenesis of lupus, phenotypic analysis of peripheral blood mononuclear cells (PBMC), NK cells cytotoxicity and cytokines production pattern of SLE patients and normal controls were examined. In addition, the effect of a variety of cytokines on anti-dsDNA antibodies production was also investigated. Our results showed that: (a) there was an increased percentage of memory T cells and decreased percentage of NK cells in SLE patients when compared to normal controls (p＜0.05); (b) a decreased production of cytokines like γ-IFN in mitogen-stimulated PBMCs was also noted in SLE patients; (c) cytolytic activity of NK cells was markedly reduced in SLE patients (p＜0.05); (d) spontaneous secretion of IgG anti-dsDNA antibodies by B cells isolated from SLE patients could be inhibited by γ-IFN, but not by IL-2, IL-4 and IL-S. These data suggested that decreased functions of NK cells and related type I T helper cells be closely related to the immune dysregulation and autoantibodies production in SLE.

並列關鍵字

syetemic lupus erythematosus ； natural killer cells ； T helper cells ； cytokines