Tumor Necrosis Factor-α Gene Promoter Polymorphism in Chinese Patients with Systemic Lupus Erythematosus

有紅斑性狼瘡的中國人腫瘤壞死因子基因啟動子的多型性變化

黃麗雪(Li-Hsueh Huang) ； 黃春明(Chung-Ming Huang) ； 蔡輔仁(Fuu-Jen Tsai)

《Formosan Journal of Rheumatology》 19卷3&4期 (2005/12) Pp. 101-106

https://doi.org/10.6313/FJR.2005.19(3-4).04

摘要

研究目的主要是檢查TNF-α啟動子－308基因的多型性對在台灣有紅斑性狼瘡的中國人是否是一個具有敏感度或評估疾病嚴重度的標記。檢查了253個健康人及84個紅斑性狼瘡病人的記錄。使用以聚合醃連鎖反應為基礎的限制型分析（Nco Ⅰ核酸內切醇），以117-bP (GG)為不反應長度及二個片段97-bP和20-P為反應長度(AA)。我們評估TNF-α啟動子－308基因與紅斑性狼瘡的相關性。對於TNF-α啟動子－308基因多型性的基因型，在紅斑性狼瘡病人與健康人為控制組間並沒有統計上明顯差異(P=0.47)。另外，我們並沒有偵測到TNF-α基因型與紅斑性狼瘡病人臨床及實驗室值有任何的相關性。

關鍵字

腫瘤壞死因子－α ； 基因多型性 ； 紅斑性狼瘡 ； 單一核苷酸多型性

並列摘要

The purpose of this study was to examine whether the tumor necrosis factor-α(TNF-α) promoter -308 gene polymorphism was a marker for susceptibility to or severity of SLE in Chinese patients in Taiwan. The records of 253 healthy people and 84 patients with SLE were examined. Using PCR-based restriction analysis (Nco Ⅰ endonuclease), we evaluated the relationship between TNF-α promoter -308 gene polymorphisms and SLE, where an unexcitable length was determined to be 117-bp (GG) and the two fragments measuring 97-bp and 20-bp were determined to be excisable lengths (AA). For the genotype of TNF-α promoter -308 polymorphism, no statistically significant differences between the SLE patient group and healthy control group (p=0.47) were found. Additionally, we did not detect any association of TNF-α genotype with the clinical and laboratory profiles in SLE patients.

並列關鍵字

TNF-α ； gene polymorphism ； SLE ； single nucleotide polymorphisms SNPs