Phytoplankton Pigment Analysis by HPLC and Its Application in Algal Community Investigations

高效液相層析法分析浮游植物色素之應用—浮游植物群聚分布研究

李秀萍(Hsiu-Ping Li) ； 龔國慶(Gwo-Ching Gong) ； 熊同銘(Tung-Ming Hsiung)

《Botanical Bulletin of Academia Sinica》 43卷4期 (2002/10) Pp. 283-290

https://doi.org/10.7016/BBAS.200210.0283

摘要

本文建立了以高效液相層析法，分析海水樣本中所含色素的方法，光合作用的主要色素，葉綠素甲及葉綠素乙之絕對偵測極限值分別為0.29和與0.27ng。本方法有效的鑑定出實驗室培養之矽藻、藍綠藻和綠藻的主要色素，三種藻類所含之葉綠素甲與其相對應的指標色素藻褐素、玉米黃質和葉綠素乙的比值，分別為1.39、0.51、1.01。以東海陸棚夏天海水樣本的指標色素分析結果，配合前述比值，本文進行了三種浮游植物群聚分布比的估算。將本方法所得之分布比與顯微鏡法的觀測結果比較，兩種方法的矽藻估算值，差異百分比小於6%；但藍綠藻的估算則發現，本方法所得分布比小於顯微鏡觀測結果。

關鍵字

浮游植物 ； 指標色素 ； 高效液相層析法 ； 群聚

並列摘要

A high performance liquid chromatography (HPLC) system for pigment analysis of marine phytoplankton was established. The characteristic quantification limits of the major photosynthetic chlorophylls a and b were 0.29 and 0.27 ng, respectively. The reliability of this method was verified by resolving the marker pigments in culture extracts from diatoms, blue-green algae and green algae. Fucoxanthin, zeaxanthin and chlorophyll b were selected for calculating the chlorophyll a/marker pigment ratios for the three types of algae, which were 1.39, 0.51 and 1.01, respectively. The composition of the phytoplankton community in summer samples, collected from the continental shelf of the East China Sea, was estimated by simple regression of these ratios. When results of composition abundance of diatoms and blue-green algae obtained by this method were compared with those obtained by the microscopic technique, the relative percentage difference was less than 6% for diatoms. On the other hand, a lower abundance of blue-green algae was estimated by the HPLC method.

並列關鍵字

Algal community ； HPLC ； Marker pigments ； Phytoplankton