Copper-Induced Stress and Antioxidative Responses in Roots of Brassica Juncea L.

本文研究了銅誘導印度芥菜根系氧化逆境的時間和濃度反應。在最初4天內植株對銅的吸收很快，之後達到飽和狀態。與對照比較，營養液中添加8µM Cu導致根系生長下降50%。銅誘導根系生長的抑制與氧化逆境有關。在最初4天內，8µM Cu處理根系內H2O2含量上升1倍，但之後又下降。根系經8µM Cu處理2天，其體現內 superoxide dismutase 活力升高1倍，這種效應持續4天，之後降至對照水平。同時，根系經8µM Cu處理後 ascorbate peroxidase、guaiacol peroxidase 活力緩慢上升，4天後急劇增高，至8天時達到最大值。根系中catalase 活力在不同濃度Cu處理下一直處於抑制狀態。8µM Cu處理普遍降低根系內還原和氧化性抗壞血酸的含量。在8µM Cu處理下，根系內還原性谷胱甘肽含量開始很低，之後回升，而氧化性谷胱甘肽含量一直較高。我們還測定了非蛋白基化合物 (Non-protein thiols, NPT) ，發現8µM Cu處理能刺激根系內NPT含量上升，但後期含量又開始下降。

關鍵字

芥菜；銅；過氧化氫；抗氧化酶；蛋白巰基化合物

並列摘要

Copper induced antioxidative reactions in the roots of Brassica juncea L. were investigated in both time-and concentration-dependent manners. The rapid uptake of Cu was observed immediately after the start of treatment. Application of Cu at 8µM caused 50 percent reduction in biomass of Cu-treated roots as compared with control. Cu-induced root growth inhibition paralleled the level of root oxidative damage. Treatment with Cu at 8µM induced a twofold increase in H2O2 content during the first 4 d, but it declined to the basal level thereafter. We also observed a twofold increase in superoxide dismutase activities with 8µM Cu during the first 2 d. The stimulation lasted for 4 d and then gradually declined. Activities of both ascorbate peroxidase and guaiacol peroxidase in roots were found to be low during the first 4 d after seedling exposure to 8µM Cu, but significantly increased after that, suggesting that increased enzyme activities would be responsible for the removal of H2O2. Catalase activities were always suppressed under Cu stress. Treatment of seedlings with 8µM Cu induced general decreases in both reduced ascorbate and dehydroascorbate. The reduced glutathione content decreased at early stages of Cu treatment. However, it was restored to the level of controls thereafter. In contrast, the oxidized glutathione contents showed a progressive increase during the time of Cu treatment. The total non-protein thiol content was shown to increase during the first several days, but it declined at later stages.

並列關鍵字

Antioxidative enzymes ； Brassica juncea L. ； Copper ； Hydrogen peroxide ； Non-protein thiols

被引用紀錄

陳培亮（2010）。台灣眼翳致病機轉之研究：(I)甲基轉移酵素3b與p16INK4a轉錄起始區過度甲基化的關聯性(II)去氧核醣核酸修補基因 APE1、hOGG1及XRCC1 之基因多型性之分析〔博士論文，中山醫學大學〕。華藝線上圖書館。https://doi.org/10.6834/CSMU.2010.00144

國際替代計量

Copper-Induced Stress and Antioxidative Responses in Roots of Brassica Juncea L.

全文下載

主題瀏覽