Population Structure and Genetic Diversity of an Endangered Species, Glyptostrobus pensilis (Cupressaceae)

珍稀瀕危植物水松居群的遺傳多樣性分析

李發根(Fa-Gen Li) ； 夏念和(Nian-He Xia)

《Botanical Bulletin of Academia Sinica》 46卷2期 (2005/04) Pp. 155-162

https://doi.org/10.7016/BBAS.200504.0155

摘要

Glyptostrohus pensilis (Staunton ex D. Don) K. Koch is a critically endangered species, only distributed in South China and Vietnam. Genetic variation within and between populations was investigated using inter-simple sequence repeat (ISSR). One hundred and seventy individuals from fourteen populations representing five regions in southern and southeastern China were sampled. The results show that genetic diversity of G. pensilis is rather low: P=10.2%, Ae=1.070 and Hpop=0.058 at population level; P=24.7%, Ae=1.140 and Hsp=0.122 at species level. The results further indicate significant genetic differentiation between regions (16.10%, analysis of molecular variance [AMOVA], p＜0.005), between populations within particular regions (29.76%, P＜0.0002), and between individuals within populations (54.14%, P＜0.0002). High levels of genetic differentiation between populations were also detected based on Nei's genetic diversity analysis (51.36%), and Shannon's diversity index (52.54%). Possible reasons for the high genetic differentiation and its low level of diversity within populations are discussed. Possible in situ and ex situ conservation strategies for G. pensilis are suggested.

關鍵字

水松 ； 瀕危種 ； 遺傳多樣性 ； 保護策略

並列摘要

Glyptostrohus pensilis (Staunton ex D. Don) K. Koch is a critically endangered species, only distributed in South China and Vietnam. Genetic variation within and between populations was investigated using inter-simple sequence repeat (ISSR). One hundred and seventy individuals from fourteen populations representing five regions in southern and southeastern China were sampled. The results show that genetic diversity of G. pensilis is rather low: P=10.2%, Ae=1.070 and Hpop=0.058 at population level; P=24.7%, Ae=1.140 and Hsp=0.122 at species level. The results further indicate significant genetic differentiation between regions (16.10%, analysis of molecular variance [AMOVA], p＜0.005), between populations within particular regions (29.76%, P＜0.0002), and between individuals within populations (54.14%, P＜0.0002). High levels of genetic differentiation between populations were also detected based on Nei's genetic diversity analysis (51.36%), and Shannon's diversity index (52.54%). Possible reasons for the high genetic differentiation and its low level of diversity within populations are discussed. Possible in situ and ex situ conservation strategies for G. pensilis are suggested.

並列關鍵字

Conservation strategies ； Endangered species ； Genetic diversity ； Glyptostrohus pensilis ； ISSR inter-simple sequence repeat