The genus Pseudozyma is an anamorph that belongs to the Ustilaginales. Pseudozyma species are unusual yeast-like fungi and are most frequently isolated from plant materials. The Pseudozyma strain, BCRC 33871, was isolated from the flower of Albizia julibrissin in Taiwan. BCRC 33871 was identified as P. antarctica and described based on morphological, physiological, and molecular data. This discovery marked the first finding of this species in Taiwan. To ensure correct identification, the intraspecific variations of P. antartica were illustrated based on physiological characteristics and phylogenetic analysis of ITS1 and ITS2 rDNA sequences. Additionally, distinguishing characteristics of P. antartica and other related species were discussed.