- 期刊
以RAPD及SSR分子標誌進行樹薯種原遺傳歧異分析及種原鑑定
Genetic Diversity and Identification of Cassava Germplasm with RAPD and SSR
摘要
本研究之植物材料爲台灣目前所保存的21個樹薯(Manihot esculenta Crantz)種原品種（系），首先利用逢機增殖多型性(Random Amplified Polymorphic DNA; RAPD)分子標誌，進行樹薯種原遺傳歧異分析，以探討各種原間之親緣關係。之後再進一步加以簡單序列重複(Simple Sequence Repeat; SSR)分子標誌進行種原鑑定，主要目的在於期望能將21個樹薯品種（系）進行完全地區分鑑別。RAPD試驗結果所獲得的156條多型性片段，經群集分析，21個樹薯種原可區分成爲4群，以提供育種參考。而以11組intra-SSR引子進行試驗，獲得32條多型性片段，經由這些多型性片段的組合，可成功地將21個樹薯品種（系）進行完全的區分，顯示SSR分子標誌在樹薯種原鑑定，爲一強而有力之工具。
並列摘要
The research aimed to identify 21 cassava (Manihot esculenta Crantz) germplasm accessions collected from Taiwan. The objectives were to understand genetic diversity among cassava germplasm accessions using RAPD and to identify each of them by intra-SSR. 21 accessions of cassava germplasm were clustered to 4 groups based on 156 polymorphic bands of RAPD. From the analysis of RAPD, it could provide as references for breeders, 32 polymorphic bands from 11 SSR primers were found and the 21 cassava varieties/lines could be differentiated completely using these intra-SSR polymorphic bands. This research revealed that the intra-SSR is a powerful molecular tool for the identification of cassava germplasm.
並列關鍵字
Cassava Manihot esculenta Crantz ； Genetic diversity ； Germplasm ； Identification ； RAPD ； SSR延伸閱讀
- 陳哲仁、周明燕、張惠如、周佳霖、鍾文全（2015）。日本及大陸地區基因改造木瓜PRSV-YK品系定量檢測技術之分析。種苗科技專訊，(91)，11-14。https://www.airitilibrary.com/Article/Detail?DocID=10219455-201509-201510190017-201510190017-11-14
- 王昭月、宋家瑋、劉明橞、歐錫坤（2007）。微衛星DNA分子標誌應用於桃栽培種與雜交後代之遺傳相似性分析。台灣農業研究，56(2)，107-120。https://doi.org/10.6156/JTAR/2007.05602.04
- 呂康祖、謝詠筌、李蕙君、劉宜祝、施養志、羅吉方（2012）。應用Nested PCR-DNA定序方法鑑定蔓荊子藥材及其製劑。食品藥物研究年報，(3)，342-347。https://doi.org/10.6945/ARFDR.201212.0342
- 陳裕星、郭俊毅、秦立德（2001）。The Analysis of Genetic Diversity of Germplasm Collections of Pea (Pisum sativum L.) by RAPD。臺中區農業改良場研究彙報，(72)，63-76。https://doi.org/10.6956/BTDAIS.200109.0063
- Ogero, K. O., Mburugu, G. N., Mwangi, M., Ombori, O., & Ngugi, M. (2012). In vitro Micropropagation of Cassava Through Low Cost Tissue Culture. Asian Journal of Agricultural Sciences, 4(3), 205-209. https://www.airitilibrary.com/Article/Detail?DocID=20413890-201205-201512280009-201512280009-205-209