- 期刊
Identification of Capsicum Chlorosis Virus Causing Chlorotic Spots and Stripes on Calla Lily
Capsicum Chlorosis Virus引起彩色海芋黃化斑點及條斑之鑑定
摘要
彩色海芋（Zantedeschia spp.）是國內重要之經濟花卉，本研究於2005年在后里地區觀察到彩色海芋植株葉片出現黃色斑點及條斑病徵。取不同罹病植株病葉經由連續三次單斑接種於奎藜（Chenopodium quinoa）後，共獲得15個純系之病毒分離株。此等病毒分離株之核酸以對應Tospovirus L基因之廣效性引子對gL3637/gL4435c進行反轉錄-聚合酶鏈鎖反應（Reverse-transcription polymerase chain reaction, RT-PCR），均可獲得與預估相符約0.9 kb之DNA片段，顯示分離株均為Tospovirus屬病毒之成員。而分離株所產生之單斑均與對應核鞘蛋白（nucleocapsid protein, NP）之抗血清包括番椒黃化病毒（Capsicum chlorosis virus, CaCV）多元抗體及西瓜銀斑病毒（Watermelon silver mottle virus, WSMoV）單株抗體產生正反應，顯示其為Tospovirus屬WSMoV血清群之成員。經全長核鞘蛋白基因序列及類緣演化分析結果，顯示此等病毒均為CaCV的分離株。進一步完成彩色海芋寄主之回接試驗，病毒分離株可於植株葉片造成與田間觀察到之類似的黃斑病徵，證實CaCV為引起此病害之病毒。
並列摘要
Calla lily (”Zantedeschia” spp.) is one of the economically important ornamental crops in Taiwan. In a field survey of calla lily conducted during 2005, plants showing symptoms of yellow spots and stripes on leaves were observed in Houli Township, one of the major areas for commercial production of ornamental crops in Taiwan. Fifteen virus isolates were collected from diseased plants of calla lily and purified via three successive local-lesion isolations on leaves of inoculated ”Chenopodium quinoa.” A 0.9 kb DNA fragment was amplified from total RNA extracted from all the fifteen virus isolates on infected plants by reverse transcription-polymerase chain reaction (RT-PCR) using the ”Tospovirus” genus-degenerate primers gL3637 and gL4510c, designed from the conserved regions of L RNA, revealing that the disease was caused by a ”Tospovirus”. The virus isolates reacted positively with the antiserum to the nucleocapsid (N) protein of Capsicum chlorosis virus (CaCV) and the monoclonal antibody to the N protein of ”Watermelon silver mottle virus” (WSMoV), indicating that they are members of the WSMoV serogroup. The nucleotide sequences of the N gene of these virus isolates from calla lily were phylogenetically related to CaCV. Furthermore, the pathogenicity of CaCV was also verified by inoculation tests on plants of calla lily.
延伸閱讀
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