利用SSR標誌進行番椒雜交種子純度之檢測

種子純度檢定是一代雜交種子生產中品質管控的關鍵性工作之一。利用生育特性的檢測 (grow out test; GOT) 方式僅比較植物生長至成熟期的外觀差異，檢測耗時且受制於環境；故亟需建立一個快速、可靠的DNA 檢測模式。本研究目的擬開發具有專一性與共顯性的簡單序列重複 (simple sequence repeat; SSR) 標誌，應用於番椒雜交種親本與雜交種子純度之檢測。利用2 個番椒雜交種及親本，篩選374 組SSR 引子對，結果獲得12 個高訊息的SSR 標誌；又取得其中5 個關鍵性SSR 標誌，進行3 個番椒雜交種 (No. 5、No. 6、No.7) 的種子純度之檢定，經各取120 個樣品之檢測結果顯示，3 批次的雜交種子純度分別為99.2%、98.3% 和95.8%。利用SSR-PCR (polymerase chain reaction) 分析可篩選出自交種子之汙染株，也與GOT檢測結果吻合。試驗顯示，SSR 標誌可作為快速、有效之檢測工具，有效地應用於番椒商業雜交種子純度的檢定。

關鍵字

番椒；簡單序列重複；雜交種子純度

並列摘要

Identification of seed purity of F_1 hybrids is a critical factor of quality control in the production of hybrid seeds. The grow out test (GOT) is based on the morphological characters of plants grown to maturity. However, GOT may influence by the environment and is time consuming, there is necessary to identify a rapid and reliable test like DNA based assay for this purpose. The objective of this study was to generate specific and co-dominant SSR (simple sequence repeat) markers for discrimination of parental lines and the subsequent assessment of seed purity in pepper hybrids. Genomic DNA from two hybrids and their parental lines were screened with 374 SSR primer pairs and 12 informative SSR markers were identified. Among the informative SSR markers, five key markers were selected for seed purity test for No. 5, No. 6, and No. 7 hybrids. A total of 120 hybrid individuals from each hybrid were tested using the identified markers, and results showed the seed purity rate for the three hybrid seed lots were 99.2, 98.3 and 95.8%, respectively. The contamination of inbred seeds obtained by SSR-PCR analysis was comparable to the GOT method. These results indicated that SSR markers could be used as a rapid and efficient tool in testing the seed purity of the commercial pepper hybrid.

並列關鍵字

Capsicum annuum ； Simple sequence repeat ； Hybrid seed purity

國際替代計量

利用SSR標誌進行番椒雜交種子純度之檢測

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