- 期刊
- OpenAccess
A Simple and Efficient Method for the Quantitative Analysis of Thymine Dimers in Cyanobacteria, Phytoplankton and Macroalgae
並列摘要
Cyclobutane dimers are the most common DNA lesions after exposure of cells to UV-B radiation. A quantitative method was developed to determine the frequency of thymine dimers in aquatic primary producers such as cyanobacteria, phytoplankton and macroalgae to study the effects of UV radiation. Genomic DNA was extracted and purified by using standard biochemical and molecular biology techniques. DNA was transferred to a nylon membrane in a slot or dot blot and incubated with a primary antibody (anti thymine dimer KTM53) against thymine dimers. The secondary antibody was an anti-mouse IgG (Fab specific) peroxidase conjugate. The blots were quantified in a Kodak Digital Science Image Station 440 CF by the chemiluminescence method. The calibration of the method was achieved by using the plasmid pBSK with known DNA sequence, length and number of adjacent thymine pairs. This method permits the measurement of low as well as high levels of DNA lesions in nanogram quantities of DNA. This method can be used for cultured as well as naturally occurring organisms.
並列關鍵字
antibody ； chemiluminescence ； cyanobacteria ； macroalgae ； phytoplankton ； thymine dimers ； UV radiation延伸閱讀
- Chen, S. Y., Pan, L. Y., & Lee, A. C. (2010). Evaluating the Culture Condition of Marine Microalga Tetraselmis chui and Its Role in a Tilapia-microalgae-clam Semi-recirculating Culture System on the Efficiency of Removing Total Ammonia Nitrogen in Water. 臺灣水產學會刊, 37(2), 99-109. https://doi.org/10.29822/JFST.201006.0003
- 謝英宗（2003）。A rapid and accurate method for the analysis of calcium carbonate and coarse fraction content in small amounts of marine sediment。國立臺灣博物館年刊，46()，71-78。https://doi.org/10.6548/ANTM.200312_46.0003
- Wong, K. H. (2006). Transgenic chlamydomonas reinhardtii as an experimental system to study the regulation of carotenoid biosynthesis in green microalgae [master's thesis, The University of Hong Kong]. Airiti Library. https://www.airitilibrary.com/Article/Detail?DocID=U0029-1812201200012352
- Li, M. (2011). Diversity of anammox bacteria in coastal and ocean sediments and interactions among ammonia oxidizers and nitrite reducers [doctoral dissertation, The University of Hong Kong]. Airiti Library. https://www.airitilibrary.com/Article/Detail?DocID=U0029-1812201200020500
- 丁建豪（2009）。Design of a new-type flow analysis for the simultaneous determination of silicate and phosphate and its application to ocean environmental studies〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2009.00454