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Phylogenetic Positions of Aspidisca steini and Euplotes vannus Within the Order Euplotida (Hypotrichia: Ciliophora) Inferred from Complete Small Subunit Ribosomal RNA Gene Sequences

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Summary. The small subunit rRNA (SSrRNA) genes were sequenced for the hypotrichous ciliates, Aspidisca steini and Euplotes vannus. These two genera form a monophyletic clade and branch first in the euplotid clade at a long level with strong bootstrap support in both distance matrix and maximum parsimony tree construction methods. The phylogenetic trees further suggest the postulated relationships among families within the order Euplotida that (1) the order Euplotida, represented by Uronychia, Diophrys, Euplotidium, Euplotes and Aspidisca, forms a paraphyletic group; (2) the families Euplotidae and Aspidiscidae, likely as a monophyletic clade, share a common ancestor; (3) two other ”related” genera, Uronychia and Diophrys, which were usually placed in the family Uronychiidae, branch later and share closer relationship each other than they are to other euplotids. On the contrary, Euplotidium arenarium, placed in the family Gastrocirrhidae, might be more closely related to Uronychia-Diophrys than to the Aspidisca-Euplotes group.

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