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Cell Surface Glycoproteins in Crithidia deanei: Influence of the Endosymbiont
並列摘要
Crithidia deanei, a protozoan of the family Trypanosomatidae harboring an endosymbiont bacterium in its cytoplasm and cured strain (endosymbiont-free) were compared as to glycoprotein composition. The wild strain of C. deanei showed a double band with molecular mass of 70/74 kDa. These bands were absent from symbiont-free cells. Indirect immunofluorescence microscopy using specific antibodies against the 70/74 kDa glycoprotein isolated from the symbiont-containing cells revealed intense labeling of the cell surface in the symbiont-harboring C. deanei. However, no such labeling was observed in symbiont-free cells. These observations suggest that the endosymbiont influences the composition of the glycoprotein on the cell surface of C. deanei.
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