Studies on the Effectiveness of Tanacetum parthenium against Leishmania amazonensis

Tatiana Shioji Tiuman ； Tânia Ueda-Nakamura ； Benedito Prado Dias Filho ； Diógenes Aparício Garcia Cortez ； Celso Vataru Nakamura

《Acta Protozoologica》 44卷3期 (2005/09) Pp. 245-251

並列摘要

We assessed the biological activity of a plant powder, crude extracts, and several fractions obtained from Tanacetum parthenium on Leishmania amazonensis. The medicinal plant T. parthenium is indicated for prevention of migraine headache crises, and several investigations have already demonstrated its anti-inflammatory activity. This study included the extraction process and bioassay-guided fractionation by the adsorption chromatography method. A progressive increase in the antileishmanial effect was observed in the course of the purification process. The plant powder (PTP) had a 50% inhibitory concentration (IC50) at 490 μg/ml, whereas the dichloromethane fraction (DF) showed an IC50 of 3.6 μg/ml against the growth of promastigote forms after 48 h of culturing. In axenic amastigote forms, the IC50 of the PTP and DF were 74.8 μg/ml and 2.7 μg/ml, respectively. Cytotoxicity analysis indicated that the toxic concentrations of the PTP, ethyl-acetate crude extract (ECE), and DF were much higher for J774G8 macrophages than for the protozoans. Haemolytic experiments were performed, and the ECE and DF did not cause lysis at concentrations higher than the IC50 for promastigotes.

並列關鍵字

antileishmanial activity ； cytotoxicity ； Leishmania amazonensis ； medicinal plants ； Tanacetum parthenium