A serological survey of brucellosis of beef cattle was conducted from July 1992 to June 1993 in Taiwan. Sera collected from 121 water buffalo and 157 yellow cattle in abattoir were examined. The sampling rates were 0.65% and 1.1% respectively, and the results were all negative. Two of 2,776 imported beef cattle sera, with the sampling rate of 13.8%, were positive in serologic reactions; the tube agglutination test titers were 1:80 or over, and the complement fixation test titers were all above 1:40. Experimental results confirmed the presence of brucellosis in beef cattle in Taiwan.