乳牛結核病檢驗方法之評估

Evaluation of the Diagnostic Methods for Bovine Tuberculosis in Dairy Herds

陳嘉榮(Chia-Jung Chen)

指導教授 ： 吳永惠

國立屏東科技大學/農學院/獸醫學系所/碩士(2008年)

https://doi.org/10.6346/NPUST.2008.00064

摘要

為評估乳牛各種牛結核病檢驗方法之實用性，對皮內結核菌素試驗 (intradermal tuberculin test, ITT) 陽性場16場87頭及陰性場8場40頭乳牛，於ITT檢驗後2週內，採血以市面新開發之Mycobacterium tuberculosis complex核酸探針冷光儀偵測法 (reliable assay protocol for identification of diseases - bioactive amplification with probing for Mycobacterium tuberculosis complex, RAPID BAP-MTB) 和Mycobacterium bovis抗體酵素免疫結合吸附法 (enzyme - linked immunosorbent assay for Mycobacterium bovis antibody, Bovine TB Ab-ELISA)，以及傳統之迦瑪干擾素試驗 (gamma interferon assay, INF-γ assay) 和巢式聚合酶連鎖反應 (Nested-polymerase chain reaction, Nested - PCR) 進行檢驗，採鼻黏液行Nested-PCR及RAPID BAP-MTB，並對ITT陽性牛採咽背和/或縱膈淋巴結行M. bovis分離與鑑定，然後評估各方法之敏感性、特異性、Kappa值和實用性。結果，若以M. bovis分離鑑定結果為判定基準，則由Kappa值顯示血液樣本Nested-PCR之敏感性 (70.6 %) 和特異性 (77.8 %)，比其他檢驗法 (Bovine TB Ab-ELISA、INF-γ assay及RAPID BAP-MTB)，呈現最為優異；若以各檢驗法為判定基準，則顯示ITT之敏感性 (54.0-100 %) 優於其他各法，但其特異性 (31.4-47.1 %) 則除優於Bovine TB Ab-ELISA 法外，普遍相對地劣於其他檢驗法 ; 又以各檢驗法為判定基準，則ITT併用Bovine TB Ab-ELISA或INF-γ assay，比單用ITT可普遍地且明顯地提高敏感性，但其特異性則相對地下降。

關鍵字

牛結核病 ； 牛分支桿菌 ； 牛結核病之診斷方法

並列摘要

In order to evaluate the practicality of diagnostic methods for bovine tuberculosis in dairy herds, the new commercial methods (reliable assay protocol for identification of diseases-bioactive amplification with probing for Mycobacterium tuberculosis complex, RAPID BAP-MTB; enzyme-linked immunosorbent assay for Mycobacterium bovis antibody, Bovine TB Ab-ELISA) and traditional methods (intradermal tuberculin test, ITT ; nested-polymerase chain reaction, Nested-PCR ; gamma interferon assay, INF-γ assay) were simultaneously conducted in 87 cows of 16 tuberculosis-infected herds and 40 cows of 8 tuberculosis-free herds within two weeks after intradermal tuberculin test (ITT). The Nested-PCR and RAPID BAP-MTB were also conducted for nasal mucus samples, and isolation of the retropharyngeal and/or mediastinal lymph nodes for M. bovis were also performed for the ITT positive cattles. Then, the sensitivities, specificities and Kappa values were evaluated. If using the results of M. bovis isolation as the diagnostic standard, the blood Nested-PCR showed the most excellent sensitivity (70.6 %) and specificity (77.8 %) than the other methods (Bovine TB Ab-ELISA, INF-γ assay and RAPID BAP-MTB) according to the Kappa values ; If using the results of each method as the diagnostic standard, ITT exhibited the most excellent sensitivities (54.0-100 %) than the other methods, but its specificities were universally lower (31.4-47.1 %) than other methods, besides the Bovine TB Ab-ELISA ; If using the results of each method as the diagnostic standard, the sensitivities of ITT combining with Bovine TB Ab-ELISA or INF-γ assay were universally and remarkably improved than ITT used alone, but their specificities were relatively decreased.

並列關鍵字

Bovine tuberculosis ； Mycobacterium bovis ； Diagnostic methods for bovine tuberculosis

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