Antigen Presenting Function of Maedi-visna Virus Infected Macrophages

MAEDI VISNA病毒感染對巨噬細胞抗原呈現功能之影響

李維誠(Wei-Cheng Lee) ； Ian McConnell ； Barbara A. Blacklaws

《中華民國獸醫學會雜誌》 23卷3期 (1997/06) Pp. 256-264

https://doi.org/10.7009/JTCSVS.199706.0256

摘要

巨噬細胞為maedi visna virus之主要標的細胞，亦具有抗原處理及呈現功能以激起免疫反應，此病毒與巨噬細胞之雙向關係，對本病之致病機轉扮演著非常重要之角色。為探索maedi visna virus感染後，對抗原呈現功能之影響，兩株抗原特之T細胞（ovalbumin及PPD-specific T cells），被使用作為本試驗之反應淋巴細胞。由培養血液衍生之巨噬細胞（monocyte-derived-macrophages），表現極低之MHC class II分子，但仍能有效呈現抗原，而激起對抗原特異之T淋巴細胞之增殖反應。巨噬細胞在maedi-visna virus嚴重感染情況下（感染後3-5天），明顯降低巨噬細胞對抗原呈現能力，同時亦降低Con A上清液在激起巨噬細胞MHC class II分子之再表現能力。

關鍵字

巨噬細胞 ； 抗原呈現功能 ； Maedi visna virus

並列摘要

Macrophages are primarily targets for maedi-visna virus (MVV) and also process and present antigen to initiate immune responses which are important in pathogenesis in lentiviral infection. To address the alteration of antigen presenting function of macrophages after infected with MVV, antigen specific T-cell lines generated from antigen primed sheep were used as responding cells. Cultured monocyte-derived-macrophages could efficiently present antigens to antigen specific T cell lines, even though they express low levels of MHC class II antigen. Productively MVV-infected monocyte-derived-macrophages showed a decrease in their antigen presenting capacity (3-5 days postinfection) and also decreased MHC class II re-expression after treated with ConA supernatant.

並列關鍵字

Antigen presenting function ； Maedi visna virus ； T cell line monocyte-derived-macrophage