lCR株小鼠犬蛔蟲症體液性免疫及抗原交又反應之研究

Humoral Immune Responses and Cross Reactions of Sera from ICR Strain Mice Infected with Toxocara canis, Ascaris suum or Angiostrongylus Cantonensis to Toxocara canis Antigens

范家堃(Chia-Kwung Fan) ； 蘇霩靄(Kua-Eyre Su) ； 鍾文政(Wen-Cheng Chung) ； 廖建維(Chien-Wei Liao) ； 藍弘旭(Horng-Shu Lan) ； 高定昌(Ting-Chang Kao) ； 蔡裕仁(Yu-Jen Tsai) ； 盧盡良(Jin-Lian Lu)

《中華民國獸醫學會雜誌》 25卷2期 (1999/06) Pp. 127-138

https://doi.org/10.7009/JTCSVS.199906.0127

摘要

本實驗以ICR株小鼠做為犬蛔蟲保幼宿主的動物模式，藉酵素標幟免疫法（ELISA）探討於感染犬蛔蟲後第一、二、三、四、六和八週時，感染型血清中IgG、IgM和IgE抗體對犬蛔蟲感染型幼蟲排泌性抗原（TcES）、幼蟲體抗原（TcLA）和犬蛔蟲成蟲體抗原（TcAA）的體液性免疫反應。結果顯示，對TcES的反應，犬蛔蟲血清中IgG抗體在感染後第二週即可測得，至第六週達到高峰。對TcLA的反感情況亦類似對TcES的作用精形。IgM及IgE抗體對TcES和TcLA反應分別於感染後第一和三週被測得。犬蛔蟲抗體參與對TcES作用的IgG亞型，主要出現在感染後第三週，為IgG2及IgG3；對TcLA 作用的IgG亞型，除第三感染週組出現IgG1及IgG2外，其餘陽性感染週組（第六及八週），皆屬IgG1。對TcAA的反應情形，則只有IgM抗體可被測得。以感染豬蛔蟲或廣東住血線蟲兩者的血清對TcES、Tc LA和TcAA交叉反應的研究顯示，對TcES的作用，兩者皆偵測則不到IgG及IgM抗體；對於TcLA的作用，僅前者血清中的IgM抗體可與之發生交叉反應；對於TcAA的作用，前者血清中IgM抗體與後者的IgG抗體可與之發生交叉反應。另外兩者血清中IgE抗體皆可和TcES與TcLA作用。本研究結果顯示同源或異源的IgM及IgE抗體對TcES、TcLA或TcAA抗原皆可出現非特異性或交叉反應。但不論以TcES或TcLA作為ELISA的被覆抗原，皆是良好的犬蛔蟲症免痠診斷性抗原，若應用於流行病學調查上，則以偵測血清中抗犬蛔蟲的IgG抗體特異性較佳。

關鍵字

犬蛔蟲症 ； 體液性免疫反應 ； 酵素標幟免疫法 ； 抗原交叉反應 ； ICR株小鼠

並列摘要

The ELISA method using Toxocara canis larval E/S (TcES) products, homogenized larval (TcLA) extracts and adult worm (TcAA) extracts, was used to evaluate the responses of IgE, IgM and IgG antibodies in sera from ICR strain mice inoculated with 250 T. canis embryonated eggs. Heterologous sera from mice infected with Ascaris suum or Angiostrongylus cantonensis were also included in the study, to assess the cross-reaction of T. canis antigens. Results revealed that homologous IgG and IgM antibodies against the TcES or TcLA antigen could be detected at the second and the first week post infection, respectively. No cross-reaction of heterologous IgG or IgM antibody against the TcES antigen was observed. Except in the case of the IgM antibody from mice infected with A. cantonensis, neither T. canis nor A. suum IgM antibody reacted with the TcAA antigen. The subclasses of homologous IgG antibodies against the TcES antigen were mainly IgG2 and IgG3. In contrast, the subclasses of homologous IgG antibodies against the TcLA antigen were mainly IgGl and IgG2. However, homologous and heterologous IgM and IgE antibodies reacted nonspecifically with TcES, TcLA or TcAA antigen thus were not satisfactory diagnostic antibodies for murine toxocariasis. Our results indicated that both TcES and TcLA antigens were excellent diagnostic antigens for murine toxocariasis by IgG-ELISA.

並列關鍵字

Toxocara canis ； Humoral immunity ； Elisa ； Antigen cross reaction ； ICR strain mice