6-氨基己烯酸對感染廣東住血線蟲鼷鼠腦脊液中纖維蛋白溶?′〝坁獐v響

Influence of epsilon aminocaproic acid on serine proteinase activity in CSF of mice infected with Angiostrongylus cantonensis

張祚愷(Tso-kai Chang)

指導教授 ： 顏全敏

高雄醫學大學/醫學院/醫學研究所/碩士(2008年)

摘要

將BALB/c鼷鼠感染三十隻廣東住血線蟲第三期幼蟲後分為給予6-氨基己烯酸的治療組及不給藥的對照組，分別觀察至感染後第三十天，發現前者之存活率較高、腦脊髓液中total leukocyte數量較少，並有顯著差異；嗜伊紅性球數量較少；白蛋白濃度較低，並且達到顯著差異；回收蟲數較少，並且達到顯著差異。而於感染後第十二、十八、二十四天之回收蟲數也是給予6-氨基己烯酸之鼷鼠較少，並且有顯著差異。而於感染蟲數不同的試驗中，腦脊髓液中total leukocyte數量、嗜伊紅性球數量及白蛋白濃度，以及回收蟲數，皆是給予6-氨基己烯酸之鼷鼠的數量較低，且total leukocyte數量及白蛋白濃度於感染四十隻蟲達到顯著差異，嗜伊紅性球及回收蟲數於感染二十隻及三十隻蟲達到顯著差異。感染十五天後始給予6-氨基己烯酸之鼷鼠其回收蟲數低於未給藥之鼷鼠，並達到顯著差異。由此發現給予6-氨基己烯酸可以降低感染的程度，並且於發炎反應高峰期過後恢復的情形較好。
給予6-氨基己烯酸之鼷鼠腦脊髓液中PAI-1濃度高於未給藥之鼷鼠，並於感染後第十二、十八、二十四及三十天達到顯著差異，tPA濃度則是未給藥之鼷鼠於感染後第十二天及第十八天較高，並於第十二天達到顯著差異。由此得6-氨基己烯酸於鼷鼠體內有抑制纖維蛋白溶?′〝坁漣@用。綜合以上推論給予6-氨基己烯酸有減輕病症效果。

關鍵字

廣東住血線蟲 ； 6-氨基己烯酸 ； 纖維蛋白溶?

並列摘要

BALB/c mice infected with 30 Angiostrongylus cantonensis L3 larvae and then treated with the plasmin inhibitor, epsilon-aminocaproic acid (EACA), might develop longer survival days, less total leukocyte counts, less eosinophil counts and less albumin concentration in the cerebral spinal fluid (CSF) and less worms recovered from the brain than those of untreated mice at 30 days after infection. There were significant difference between treated and untreated mice in total leukocyte counts and albumin concentrations in CSF at 30 days after infection (P<0.05), and in the recovery of worms from brain at 12, 18 and 24 days after infection (P<0.05). All groups of mice infected with different numbers of L3 larvae and then treated with EACA might develop less total leukocyte counts, less eosinophil counts and less albumin concentration in the CSF and less worms recovered from the brain than those groups of untreated mice at 21 days after infection. There were significant difference between treated and untreated mice in total leukocyte counts and albumin concentration in CSF of mice infected with 40 larvae (P<0.05); eosinophil in CSF and worms recovered from brain in mice infected with 20 and 30 larvae were also significant difference between treated and untreated groups (P<0.05). Mice began to be treated with EACA from 15 days after infection might develop significantly less worms recovery compared with untreated mice (P<0.05).
BALB/c mice, infected with 30 Angiostrongylus cantonensis L3 larvae and then treated with EACA, developed higher PAI-1 levels in CSF on days 12, 18, 24 and 30, and less tPA levels in CSF on days 12 and 18 days after infection. There were significant difference between treated and untreated mice in PAI-1 levels on days 12, 18, 24 and 30 (P<0.05), and in tPA levels at 12 days after infection (P<0.05). In this study, those results indicate that EACA could palliate clinical symptoms of angiostrongyliasis mice and infected mice were convalescently well after severe inflammation.

並列關鍵字

Angiostrongylus cantonensis ； epsilon aminocaproic acid ； serine proteinase activity

參考文獻

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