Potency Determination of Cell Culture Derived Japanese Encephalitis Vaccine in Horses Against Virus Challenge

本研究的目的，以馬匹免疫實驗，評估細胞產製日本腦炎疫苗的效價。此種不活化日本腦炎精製疫苗，以非洲綠猴腎細胞株做為病毒增殖的基質，經由液相層析純化後精製而得。在第一個實驗中，分別在第1週、第3週及第10週作免疫接種。其中1.25及5倍相對抗原單位之兩實驗組，在接種後13週，發現其中和抗體力價為1:2046和1:3625，並沒有明顯的區別。另一組接種5劑量的現行人用NIPM鼠腦產製日本腦炎疫苗，則無法誘發中和抗體力價達1:10以上。於第二個實驗中，則採行間隔四週注射兩劑之方式，分別施打市售馬用日本腦炎疫苗（日本日生研製造）與此精製疫苗。結果接種1.5，3及6倍相對抗原單位之實驗組，其保護力價，皆較市售馬用疫苗為佳，精製疫苗誘發中和抗體之力價可高達市售馬用疫苗之20倍。但此四組實驗組在免疫13週後，皆能保護馬匹承受日本腦炎病毒的攻擊。此次馬匹免疫實驗在一九九九年一月至八月間進行，陰性對照組在六月時因自然感染有抗體產生，此現象與台灣日本腦炎流行季節相符。由上述結果顯示，細胞產製日本腦炎精製疫苗，可有效控制日本腦炎病毒對馬的感染。

關鍵字

日本腦炎；疫苗；馬

並列摘要

Potency measurements of cell culture derived Japanese encephalitis (JE) vaccines were performed in horses that were anti-JE virus antibody free. The inactivated vaccine was prepared from infected Vero cells substrates after purification by liquid chromatography. In program Ⅰ, inoculations were introduced at the first, third and tenth weeks. Neutralizing antibody responses for groups with antigen contents of ELISA units 1.25 and 5 were about equal with average of 1:2046 and 1: 3625 at 13-week post-vaccination. On the other hand, neutralizing antibody titers induced by NIPM JE vaccine (5 human doses), prepared from infected adult mouse brain, were detected below 1:10. In program Ⅱ, vaccines were injected two times at weeks 1 and 5. The virus neutralization antibody response turned out to be greater in groups that received three different dosages, antigen contents of ELISA units 1.5, 3, and 6, of cell culture derived JE vaccine respectively, as compared to another group received a commercial available veterinary JE vaccine (Nisseiken Company) for horses from Japan. The neutralizing antibody titer was 20 times higher. The results, however, obtained in horses that were challenged at 13-week post-vaccination, demonstrated that inoculation of both cell culture derived JE vaccine and the commercial equine vaccine generated a protective immune response to prevent viremia. These horses were conducted from January to August 1999. The time of seroconversion in the placebo group was June. The phenomenon conformed to the epidemic season of JE in Taiwan. These results demonstrate that the newly developed cell culture derived Japanese encephalitis vaccine is an effective means to control JE disease in horses.

並列關鍵字

Japanese encephalitis ； Vaccine ； Horse

國際替代計量

Potency Determination of Cell Culture Derived Japanese Encephalitis Vaccine in Horses Against Virus Challenge

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