- 期刊
臺灣肉雞沙氏桿菌、彎曲桿菌之盛行率及抗菌劑感受性調查
Prevalence and Antimicrobial Susceptibility of Salmonella and Campylobacter from Meat-type Chickens in Taiwan
摘要
從1998年7月到1999年11月對台灣12個縣之肉雞場調查沙氏桿菌及彎曲桿菌之污染情形。採集之共泄腔拭子經增菌後，續以選擇性培養基分離沙氏桿菌，再以血清學方法鑑定血清型別，結果雞場陽性率為47.2% (51/108)，雞隻陽性率為10.2% (64/626)，其中以Salmonella schwarzengrund（32株）及S. typhimurium（19株）兩種血清型最多，占全部分離株的79.7%。所有沙氏桿菌對florfenicol、ceftiofur、nitrofurantoin等藥有較佳感受性；而ciprofloxacin對所有沙氏桿菌的最低抑制濃度MIC_(50)為0.125 μg/mL, MIC_(90)為8.0 μg/mL。共泄腔拭子並經Preston培養基在微好氧環境下分離彎曲桿菌後，經革蘭氏染色及生化試驗套組API Campy®鑑定，結果雞場陽性率為24.1% (26/108)；雞隻陽性率為7.2% (45/626)，其中以Campylobacter jejuni（30株）最多，佔66.7%。所有彎曲桿菌對florfenicol、gentamicin、nitrofurantoin等藥有較佳感受性；而ciprofloxacin對所有彎曲桿菌的MIC_(50)為0.38μg/mL, MIC_(90)則高於32 μg/mL。肉雞場之沙氏桿菌及彎曲桿菌污染情形有極顯著正相關(P＜0.001)，顯示雞場生物安全防護之重要性。
並列摘要
In 1998 and 1999, cloacal swab samples were collected from meat-type chickens (both broiler and simulative native chicken) in 12 counties in Taiwan to estimate the prevalence of Salmonella and Campylobacter infections. We found 47.2% (51/108) of the flocks and 10.2% (64/626) of the chickens were Salmonella positive. S. schwarzengrund and S. typhimurium were the most prevalent serotypes. Based on antimicrobial susceptibility tests, Salmonella isolates were most sensitive to florfenicol, ceftiofur and nitrofurantoin. The minimum inhibitory concentration (MIC_(50)) of ciprofloxacin was 0.125 μg/mL and the MIC_(90) was 8.0 μg/mL. We found that 24.1% (26/108) of the flocks and 7.2% (45/626) of the chickens were Campylobacter positive. C. jejuni was the most prevalent serotype. Campylobacter isolates were most sensitive to florfenicol, gentamicin and nitrofurantoin. For Campylobacter, the MIC_(50) and MIC_(90) of ciprofloxacin were 0.38 μg/mL and greater than 32 μg/mL, respectively. Flocks infected with Campylobacter were significantly more likely to be infected with Salmonella (P＜0.001).