豬內源性反轉錄病毒結構基因之選殖與序列分析

Molecular Cloning and Sequence Analysis of Porcine Endogenous Retrovirus

張荏婷(Jen-Ting Chang) ； 蘇莉婷(Li-Ting Su) ； 侯庭鏞(Tin-Yun Ho) ； 王仕蓉(Shih-Rong Wang)

《中華民國獸醫學會雜誌》 27卷2期 (2001/06) Pp. 94-103

https://doi.org/10.7009/JTCSVS.200106.0094

摘要

豬內源性反轉錄病毒（porcine endogenous retrovirus, PERV）是近年來使用豬隻組織醫材的一大隱憂，為了研究探討本土PERV病毒株之特性與感染機制，我們已成功地自豬細胞株（PK15和ST cell）及蘭嶼小耳種豬選殖出PERV重要結構蛋白之基因包括gag, pol及env區域；經推測gag區域之核酸序列可轉譯出512個胺基酸，pol區域可轉譯出368個胺基酸，env區域可轉譯出612個胺基酸。經比對分析發現不同來源PERV之gag-pol區域有94-98%之相似性。另以PERV-env之序列作分析證實PERV-ST, PERV-PK15和PERV-LS三種病毒株皆屬於subtype A；且與feline leukemia virus, murine leukemia virus, gibbon ape leukemia virus及murine leukemia virus有68~71%之相似性。

關鍵字

豬內源性反轉錄病毒 ； 結構蛋白 ； 序列比對

並列摘要

The potential risk of pig endogenous retroviruses (PERV) infection is critical for the future of xenotransplantation. To study the infectious mechanism of PERV, the pol, gag and env regions of porcine endogenous retrovirus (PERV) from ST cell line, PK15 cell line and Lee-Song miniature pig were isolated and characterized. The sequences of gag, pol and env regions encoded 512, 368, 612 amino acids, respectively. Nucleotide sequence analysis indicated that different isolated PERVs shared 94-98% of homology in gag-pol region. Phylogenetic analysis of env region revealed that all of PERV-ST, PERV-PK15 and PERV-LS isolates belong to type A of PERV. Additionally, the sequence of PERV isolated in our lab was highly homology (68-71%) with feline leukemia virus, murine leukemia virus, gibbon ape leukemia virus and simian sarcoma virus.

並列關鍵字

Porcine endogenous retrovirus ； Structural gene ； Sequence analysis