以豬第一號染色體微衛星型遺傳標記交替基因頻率與序列比較經產母豬產仔性能

Comparison of the litter performance of parous sows in aspects of allele frequencies and sequences of microsatellite markers on swine chromosome 1

廖仁寶(Ren-Bao Liaw) ； 黃鈺嘉(Yu-Chia Huang) ； 賴永裕(Yung-Yu Lai) ； 顏念慈(Neim-Tsu Yen) ； 吳明哲(Ming-Che Wu) ； 張秀鑾(Hsiu-Luan Chang)

《畜產研究》 39卷2期 (2006/06) Pp. 99-110

摘要

豬第一號染色體上的八個微衛星型遺傳標記SW1514、SW552、SW2185、SW1301、SW373、SW780、S0316、SW2130，在第一號染色體連鎖圖譜約佔140分摩根。本研究以八個遺傳標記分析157頭純種母豬DNA的基因型，比較不同基因型母豬之總產仔數、活仔數及仔豬存活率。每個遺傳標記之交替基因頻率，皆有品種間的差異。以最小平方法分析個別遺傳標記交替基因在產仔性能之效應：杜洛克母豬具SW1514B3交替基因（P＜0.001）和SW1301B1交替基因（P＜0.01），其總產仔數比未具有該交替基因者顯著地多。而藍瑞斯母豬具SW373B5交替基因（P＜0.01）與杜洛克母豬具SW1514B3交替基因（P＜0.01），其產仔活仔數亦顯著地比未具有者高。同樣地，不同品種母豬具有某些交替基因，其產仔性能則會比未具有該交替基因者差。選殖與定序分析SW373和SW780交替基因DNA序列，發現SW373之重複區為不同數目TG雙核苷酸重複所構成，其核苷酸重複型式可區分成四種分別為（TG）_pAG（TG）_q、（TG）_pAG（TG）_qAG（TG）_rAG、（TG）_pAG（TG）_qAG（TG）_rAGTGAG、（TG）_pAG（TG）_qG（TG）_rAG（TG）_sAGTGAG；而SW780之重複區則由不同數目的兩種雙核苷酸重複和一種四核苷酸重複而構成，其核苷酸重複型式為（GT）_x（GA）_y（GACA）_z。因此，每個交替基因變異區的核苷酸重複型式可能複雜。

關鍵字

微衛星型遺傳標記 ； 交替基因 ； 核苷酸重複

並列摘要

A total of eight microsatellite markers on swine chromosome 1 (SSC1) were used to genotype 157 parous sows. These markers including SW1514, SW552, SW2185, SW1301, SW373, SW780, S0316 and SW2130 spanned about 140 cM on the linkage map of SSC1. Three traits including the litter size at birth, the litter size born alive and the survival rate of piglets were analyzed. The genotyping analysis indicated that the allele frequency of every marker differed among four breeds. When we further analyzed the litter performance of sows with different alleles by the least-square method, it was shown that the number of total piglets born from Duroc sows with SW 1301B3 allele were significantly higher than those without such an allele, and so did SW1301B1 allele. For Landrace sows with SW373B5 allele and Duroc sows with Sw1514B3 allele, the number of born alive piglets were significantly higher than that without such an allele. In the same way, the trait of litter performance of different breed sows with some alleles was be inferior to those without such alleles. Besides, after sequencing and analyzing the allele sequences of SW373 and SW780 from five pigs, the results indicated that the tandem repeat region of SW373 was composed of different number of TG repeats and that the patterns consisted of four kinds, including (TG) _pAG (TG)_q, (TG)_pAG (TG)_qAG (TG)_rAG,(TG)_pAG(TG)_qAG (TG)_rAGTGAG, and (TG)_pAG (TG)_qG (TG)_rAG (TG)_sAGTGAG. However, the tandem repeat region of SW780 was composed of different number of two kinds of di-nucleotide repeats and one kind of tetra-nucleotide repeat, and the patterns consisted of (GT)_x (GA)_y (GACA)_z. Therefore, the nucleotide repeat pattern in variant region of each allele might the rather complicated.

並列關鍵字

Microsatellite marker ； Allele ； Litter performance