以液相層析法同步檢測吳郭魚組織中之氯黴素、氟甲磺氯黴素及甲磺氯黴素

Simultaneous Determination of Chloramphenicol, Florfenicol, and Thiamphenicol in Tilapia Tissues by HPLC

王渭賢(Way-Shyan Wang) ； 許天耀(Tain-Yao Hsu) ； 何素鵬(Shu-Peng Ho) ； 張聖宗(Sheng-Tsung Chang) ； 施清文(Chin-Wen Shih)

《中華民國獸醫學會雜誌》 27卷3期 (2001/09) Pp. 221-231

https://doi.org/10.7009/JTCSVS.200109.0221

摘要

本研究之目的在於開發一種同步檢測魚肉中氯黴素(chloramphenical, CAP)、氟甲磺氯黴素(florfenical, FFO)及甲磺氯黴素(thiamphenicol, TAP)之方法，我們利用高效液相層析法來達此目的。以裝有保護管柱(LiChrospher® 100, RP-18)之分離管(Cosmosil 5C18-MS)，移動相0.05 M ammonium acetate-acetonitrile-tetrahydrofuran (76:23:1, pH 7.2)，並配合220 nm波長檢測可同時精確地分離CAP、TAP及FFO。測試藥物TAP，FFO及CAP之滯留時間分別為5.68 min、11.95 min及16.15 min。各藥物之標準曲線相關係數相當好（r^2值可達0.998），且其最低檢測濃度可達1 ng。利用固相萃取法淨化吳郭魚組織內藥物，以去離子水清洗後，以n-hexane移除脂溶性物質及acetonitrile作為提取液，經濃縮後再以去離子水溶解而成最終檢液。結果肌肉組織之回收率為81.5-100%，最低檢測濃度為0.01 μg/g；肝臟組織之回收率為81.7-92%，最低檢測濃度為0.05μg/g。各藥物回收率之相關係數亦相當好（r^2值可達0.99以上）。

關鍵字

高效液相層析法 ； 氯黴素 ； 氟甲磺氯黴素 ； 甲磺氯黴素 ； 吳郭魚

並列摘要

The aim of this study was to develop a rapid method to detect the residues of the chloramphenical (CAP), florfenicol (FFO) and thiamphenicol (TAP) in fish meat at the same time. 0.05 M ammonium acetate-acetonitrile-tetrahydrofuran (76:23:1, pH 7.2) as the mobile phase at a flow rate of 1.0mL/min, the high performance liquid chromatographic (HPLC) method for the simultaneous determination of CAP, TAP, and FFO was carried out on a C18 column (Cosmosil 5C18-MS, 150 mm × 4.6 mm I. D.) with a guard column ( LiChrospherR 100, RP-18). The wavelength of UV detector was set at 220 nm in this investigation. The retention time of CAP, TAP and FFO were 5.68, 11.95, and 16.15 minutes, respectively. The r-square values of standard curve of drug concentration of each drug were up to 0.998. The limited detection concentration of our developed method was 1 ng for each tested agent. The residues of tested drugs in the muscle, liver and kidney of tilapia extracted by solid-phase extraction method were detected with our developed method in this study. The treated tissue was cleaned by following water and n-hexane and eluted by acetonitrile. The results revealed that the recoveries of muscle, liver and kidney from tilapia were 81.5-100.2%, 81.7-92% and 82.4-97.8%, respectively. The detection limit of muscle, liver and kidney from tilapia were 0.01, 0.025 and 0.05 μg/g, respectively. The r-square values of recovery curve of each drug were up to 0.99.

並列關鍵字

HPLC ； Chloramphenicol ； Florfenicol ； Thiamphenicol ； Tilapia