以高效液相層析法檢測確認Sulfadimethoxine投予虱目魚後之殘留量

Residual Level of Sulfadimethoxine Administered in Milkfish Determined with High Performance Liquid Chromatography

呂車鳳(Che-Feng Lu) ； 江彥德(Yann-Der Jiang) ； 陳瑞雄(Rey-Shyong Chern) ； 陳雨新(Yu-Shin Chen)

《中華民國獸醫學會雜誌》 21卷4期 (1995/08) Pp. 242-247

https://doi.org/10.7009/JTCSVS.199508.0242

摘要

本試驗探討sulfadimethoxine（磺胺二甲氧嘧啶；SDM）以100 mg/kg BW/day之劑量連續3天口服投予虱目魚，利用高效液相層析法來檢測SDM在虱目魚中殘留之情況，且以光二極體陣列式檢測器來確認該藥。依設定之抽取法及分析條件，其結果如下：SDM標準品檢測極限為0.1ppm，虱目魚肉中SDM檢測極限則為0.01 ppm。殘留試驗停藥後24小時魚肉中SDM之濃度達到最高（0.18 ppm），爾後呈下降趨勢，於第24天起已檢測不出有SDM之殘留（即低於0.01 ppm）。

關鍵字

磺胺二甲氧嘧啶 ； 高效液相層析 ； 殘留 ； 虱目魚

並列摘要

Sulfadimethoxine was given orally to milkfish at 100 mg per kg bw daily for 3 consecutive days. Then, the residue of the drug in flesh was determined with high performance liquid chromatography and confirmed with photodiode array detector. The detection limits of sulfadimethoxine in standard solution and milkfish flesh were 0.1 and 0.01 ppm, respectively. The peak residue level of 0.18 ppm appeared 24 h after withdrawing the drug and showed a decreasing gradient thereafter. No sulfadimethoxine (above 0.01 ppm) was detected in the fish flesh from 24th day to 30th day after withdrawing the drug from the fish.

並列關鍵字

Sulfadimethoxine ； HPLC ； Residue ； Milkfish