台灣欒樹葉子熱水抽出物中抗氧化及抗發炎活性成分之分離及鑑定

Isolation and Identification of Antioxidative and Anti-inflammatory Constituents from Hot Water Extracts of ＂Koelreuteria henryi＂ Leaves

曹隽瑋(Chun-Wei Tsao) ； 柯慧君(Huey-Jiun Ko) ； 蔡佺廷(Chuan-Ting Tsai) ； 杜明宏(Ming-Horng Duh)

《林產工業》 28卷4期 (2009/12) Pp. 273-284

https://doi.org/10.6561/FPI.2009.28(4).5

摘要

本研究乃對台灣欒樹（Koelreuteria henryi Dummer）葉部熱水抽出成分進行抗氧化與抗發炎活性評估。同時將其抗氧化成分分離與鑑定。台灣欒樹葉部熱水抽出物之正丁醇可溶部以管柱層析及高效能液相層析分離後可得化合物1及2，經NMR鑑定後得知分別為Gallic acid及1,3,4,5-Tetra-O-galloylquinic acid。DPPH （1,1-Diphenyl-2-picrylhydrazyl）抗氧化試驗結果顯示正丁醇可溶部次分離部Bu3 具有最佳之EC_(50)值（Half maximal effective concentration, EC_(50)）（3.3μg/mL），而化合物1和2之EC_(50)值則分別為4.3 與5.5μg/mL。細胞存活及抗發炎活性篩選試驗結果顯示，所有試驗樣品對RAW 264.7細胞株之一氧化氮生成均具抑制作用，且皆有隨濃度增加而提升之趨勢。於試驗濃度50 μg/mL時，台灣欒樹樹葉熱水抽出物及其乙酸乙酯可溶部、正丁醇可溶部及化合物1與2之抑制率IE （Inhibitory effects）值皆可達到75%以上，其中又以化合物1效果最佳，當試驗濃度為1 μg/mL時，抑制率即可達72.6%。綜合上述試驗結果，台灣欒樹葉部熱水抽出物含有抗氧化性及抗發炎性之物質，且對小鼠巨噬細胞不具毒性。

關鍵字

台灣欒樹 ； 熱水抽出物 ； 抗發炎活性 ； 五倍子酸

並列摘要

The antioxidant and anti-inflammatory activities of hot water extracts from Koelreuteria henryi Dummer leaves were investigated in this study. The major antioxidant compounds in the soluble fractions of hot water extracts were also isolated and identified. Compounds 1and 2 were isolated from n-Butanol fraction by CC and HPLC, and further identified as gallic acid and 1,3,4,5-tetra-O-galloylquinic acid, respectively, by NMR. The subfraction 3 (Bu3) from n-Butanol fraction exhibited the strongest DPPH (1,1-Diphenyl-2-picrylhydrazyl) scavenging activity with an EC_(50) value of 3.3 μg/mL. DPPH EC_(50) values of compounds 1and 2 were 4.3 and 5.5 μg/mL, respectively. The results of cell toxicity and anti-inflammatory activities assessment indicated that all tested samples inhibited LPS-induced NO production by RAW 264.7 cell in a dose-dependent manner without affecting cell viability. IE values of crude extract, ethyl acetate fraction, n-Butanol fraction, compounds 1 and 2 were＞75 % at concentration of 50 μg/mL. Compound 1 exhibited the highest anti-inflammatory activity, and its IE (Inihibitory effects) value was 72.6 % at concentration of 1 μg/mL. The results indicated that hot water extract from K. henryi leaves have antioxidation and anti-inflammatory agents and were not cytotoxic to murine macrophages.

並列關鍵字

Koelreuteria henryi Dummer ； Hot water extracts ； Anti-inflammatory activities ； Gallic acid ； 1,3,4,5-Tetra-O-galloylquinic acid