水溶性天然色素系統分析法之探討

探討濾紙層析法(Paper Chromatography)，薄層層析法(Thin-layer Chromatography)及分光光度法用於分離鑑別九種水溶性天然色素之最佳條件。結果濾紙層析法的展開溶媒以n-Butanol：Acetic acid：Water （4:1:5，取上層液）最佳，另外n-Butanol：Acetic acid：Water (4:1:2)及Isoamyl Alcohol：Acetone：Water (5:6:5)可幫助鑑別。以聚醯胺(Polyamide)為擔體之薄層層析法的展開溶媒以Isopropanol：Formic acid：Water (7:1:3)最佳。由食品中分離色素，以SEP-PAK C18 Cartridge取代傳統之聚醯胺管柱(Polyamide Column)，其方法迅速簡便，檢體所需量少且干擾少，核黃素(Riboflavin)不被聚醯胺吸附，但可被SEP-PAK C18 Cartridge吸附。配合濾紙層析法或薄層層析法，可將九種水溶性天然色素分離鑑別，效果良好。自台北市區抽購標示含天然色素之檢體三十件，調查結果：水溶性婀娜多二件，核黃素一件，銅葉錄素鈉二件，糖焦色素二件，藏花素二件。顯示本法應用於食品中天然色素之分離鑑別，結果良好。

關鍵字

水溶性天然色素；聚醯胺；薄層層析法；濾紙層析法

並列摘要

A method is described for the rapid detection of 9 water-soluble natural dyes in foods. Sample solution, prepared by aqueous extraction of foods, was shaken with ether to remove oil-soluble materials. The solution was adjusted to pH 3-4. The dyes in the solution were then adsorbed by SEPPAK C18 cartridge and eluted with 0.05% HCl in methanol. The dyes were identified by paper chromatography, thin-layer, chromatography and visible spectrophotometry. The solvent systems used for the chromatographies were as follows: paper chromatography, n-butanol: acetic acid: water (4:1:5, upper layer); polyamide thin. layer chromatography, isopropanol: formic acid water (7:1:3). This method was applied for the detection of water-soluble natural dyes in 30 samples, including candies, soft drinks and other foods. The results showed that the water-soluble dyes in the samples were: annatto (water-soluble) 2, riboflavin 1, sodium copper chlorophyllin 2, caramel 2, and crocin 2.

並列關鍵字

Water-soluble natural dye ； Polyamide ； Thin-layer Chromatography ； Paper chromatography

國際替代計量

水溶性天然色素系統分析法之探討

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