保色劑中硝酸塩及亞硝酸塩液相層析分離定量法之探討

Deternination of Nitrate and Nitrite by High Performance Liquid Chromatography in Color Fasting Agents

蘇淑珠(Shu-Ju Su) ； 林小華(Shau-Wha Lin) ； 李樹其(Shu-Chi Lee) ； 周薰修(Shin-Shou Chou)

《藥物食品檢驗局調查研究年報》 8號 (1990/09) Pp. 159-164

https://doi.org/10.6946/ASRNL.199009.0159

摘要

本定量法係採用二甲酚法，將硝酸塩、亞硝酸塩於酸性下分別與2,6-二甲酚反應，就所得生成物4-硝基－2,6-二甲酚，4-亞硝基－2,6-二甲酚以氯仿抽出後，再利用高效液相層析法分離定量。其高效液相層析條件為，以μ-Porasil (10 μm, 4mm I.D.×250mm)層析柱為固定相，以氯仿一醋酸(99.5:0.5)為移動相，檢出器之紫外光波長為310nm。
就市售保色劑及市售肉製品利用本定量法進行檢測，結果以市售保色劑普德粉之配方製成之檢體作回收實驗時，所得平均回收率為硝酸鉀99.1%，亞硝酸鈉99.54%，至於食品部份，由香腸檢體作回收實驗，所得平均回收率為硝酸塩95.0~101.0%，亞硝酯塩99.0~100.0%，本方法之檢出限量為NO3-N 0.2μg/ml，NO2-N 0.02 μg/ml，為一精確迅速、簡便可行之分析方法。

關鍵字

高效液相層析法 ； 硝酸塩 ； 亞硝酸塩 ； 二甲酚法 ； 4-亞硝基－2,6-二甲酚 ； 4-亞硝基－2,6-二甲酚

並列摘要

Determination of nitrate and nitrite using xylenol method was studied. It is based on the formation of 4-nitro-2,6-xylenol by the reaction of nitrate with 2,6-xylehol, and the formation of 4-nitroso-2, 6-xylenol by the reaction of nitrite with 2, 6-xylenol. These two products were extracted with chloroform and simultaneously analyzed by mesans of high performance liquid chromatography with a UV detector (310nm) and a μ-porasil column with chloroform: acetic acid (99.5:0.5) as the mobile phase. The recoveries from sausage were 95.0~101.0% for nitrate and 99.0~100.07 for nitrite. The detection limits for nitrate nitrogen and for nitrite nitrogen were 0.2 and 0.02μg/ml, respectively. The methed is rapid and the results were reproducible.

並列關鍵字

High Performance Liquid Chromatography ； Nitrate ； Nitrite ； Xylenol method ； 4-Nitro-2.6-xylenol ； 4-Nitroso-2,6-xylenol