罐裝飲料中丙二酚A及丙二酚A二環氧甘油醚檢驗方法之建立

Quantitative Assay of Bisphenol A, Bisphenol A Diglycidyl Ether in Canned Drinks

張美華(Mei-Hua Chang) ； 李凰绮(Huang-Chi Lee) ； 高雅敏(Ya-Min Kao) ； 周薰修(Shin-Shou Chou)

《藥物食品檢驗局調查研究年報》 25號 (2007/12) Pp. 293-301

https://doi.org/10.6946/ASRNL.200712.0293

摘要

丙二酚A(bisphenol A, BPA)、丙二酚A二環氧甘油醚(bisphenol A diglycidyl ether, BADGE)及其衍生物，包括2種水合物丙二酚A二環氧甘油醚單水合物(bisphenol A(2,3-dihydroxypropyl)glycidyl ether, BADGE•H2O)、丙二酚A二環氧甘油醚二水合物(bisphenol A bis(2,3-dihydroxypropyl)ether, BADGE•2H2O)及3種含氯化合物丙二酚A二環氧甘油醚氫氯化物(bisphenol A(3-chloro-2-hydroxypropyl) glycidyl ether, BADGE•HCl)、丙二酚A二環氧甘油醚二氫氯化物(bisphenol A bis(3-chloro-2-hydroxypropyl) ether, BADGE•2HCl)和丙二酚A二環氧甘油醚水合物氫氯化物(bisphenolA(3-chloro-2-hydroxypropyl)(2,3-dihydroxypropyl) ether, BADGE•H2O•HCl)，為罐頭塗料溶出物，本研究使用高效液相層析法配合螢光檢出器建立罐裝飲料其含量之檢測方法。依飲料類型，檢體分別以甲基第三丁基醚或乙腈萃取，經乙腈飽和之正己烷去油脂、再以Sep-Pak C18及Sep-Pak Florisil過濾層析匣淨化、或濾膜過濾，以高效液相層析儀分析定量。使用之層析管柱Luna C18(2) (25 cm × 4.6mm i.d., 5 μm thickness)，以水/乙腈/甲醇梯度移動相溶液，於激發波長230 nm、放射波長304 nm可同時分離檢測7種化合物。添加0.2、0.4及0.8 ppm混合標準溶液於咖啡檢體中，其平均回收率為80.9∼108.8%，變異係數皆小於5.5%；添加0.05、0.1及0.2 ppm混合標準溶液於蕃茄汁檢體中，其平均回收率為81.1∼109.8%，變異係數皆小於5.7%。本方法之檢出限量BADGE•2H2O、BADGE•H2O•HCl及BADGE•2HCl均為0.003 ppm，BPA、BADGE、BADGE•H2O及BADGE•HCl均為0.005 ppm。

關鍵字

丙二酚A ； 丙二酚A二環氧甘油醚 ； 高效液相層析

並列摘要

Bisphenol A(BPA), bisphenol A diglycidyl ether(BADGE) and its derivatives, including 2 hydrolyzed products, bisphenol A(2,3-dihydroxypropyl) glycidyl ether(BADGE•H2O) and bisphenol A bis(2,3-dihydroxypropyl) ether(BADGE•2H2O), and 3 chlorohydrin products, bisphenol A(3-chloro-2-hydroxypropyl) glycidyl ether(BADGE•HCl), bisphenol A bis(3-chloro-2-hydroxypropyl) ether(BADGE•2HCl), and bisphenol A(3-chloro-2- hydroxypropyl)(2,3- dihydroxypropyl) ether(BADGE•H2O•HCl), can dissolve out of can coatings into food. A quantitative method was developed to assay these compounds in canned drinks by high performance liquid chromatography(HPLC) coupled with a fluorescence detector. Depending on the drink types, these compounds were extracted using tert-butyl methyl ether or acetonitrile, defatted with n-hexane, cleaned up with Sep-Pak C18 and Florisil columns, and then analyzed by HPLC. The chromatographic separation was effected with gradient elution of water, acetonitrile and methanol on a Phenomenex Luna C18(2) column. Recovery analysis was performed by spiking standard compounds into tomato juice at 0.05, 0.1 and 0.2 ppm levels and coffee drinks at 0.2, 0.4 and 0.8 ppm levels. Average recoveries were higer than 80% and the coefficients of variation were less than 5.7%. The detection limits of method were 0.003 ppm for BADGE•2H2O, BADGE•H2O•HCl and BADGE•2HCl, and 0.005 ppm for BPA、BADGE、BADGE•H2O and BADGE•HCl.

並列關鍵字

bisphenol A ； bisphenol A diglycidyl ether ； high performance liquid chromatography