臺灣山櫻花APETALA1基因之選殖與序列分析

花器發育在植物繁衍後代的過程中扮演關鍵的角色，在其發育過程中，APETALAI（API）基因為調控花發育最重要的基因之一，此基因主要誘導萼片且花瓣的發育，另外此基因亦調控花分生組織轉變。我們自資料庫中蒐集並且分析已發表之開花植物API序列，在具高保守性的序列中設計一對退化性引子，對山櫻花（Prnus campanuIata Maxim.）進行API的釣取，並同時配合RACE（Rapid Amplify of eDNA End）技術，成功獲得山櫻花之API全長，並將其命名為PcAPI。對此基因進行分析比對後，其結果顯示，PcAPI和所有已知之此類基因相同，具有高度保守之MADS-box，並且與薔薇科植物之API在核酸序列且蛋白質序列上皆具極高之相似性，另外此序列資料亦可進行蛋白質功能區塊和蛋白質模型之預測，有助於更深入了解山櫻花之開花基因於植物體內如何作用。

關鍵字

山櫻花；花器發育； ABC模型

並列摘要

Flower development plays a key role in the process of plant reproduction. APETALA1 (AP1) gene is one of the most important genes involved in the development, and this gene regulates normal development of sepals and petals. Furthermore, AP1 gene also specifies flower meristem identity. In order to get the fragments of AP1 gene, we designed a pair of degenerated primers based on the conserved region of AP1 gene from several different plants, and then used it with single-stranded cDNA form Prnus campanulata by polymerase chain reaction (PCR). The full-length gene, named as PcAP1, was retrieved after Rapid Amplification of cDNA Ends (RACE) process. The cloned gene was sequenced and the amino acid sequence was predicted. Comparing the alignment data of PcAP1 amino acid sequence with other plants AP1 gene, they are identical with highly conserved MADS-box. Furthermore, the PcAP1 gene cloned from P. campanulata is highly similarity with the same gene from other Rosaceae plants. This research also provides valuable information for predicting the protein function motifs and protein structure of this gene. Further research may help us to have better understanding about how the AP1 gene works in plant flowering.

並列關鍵字

Prnus campanulata ； flower development ； AP1 ； MADS-box ； ABC model

國際替代計量

臺灣山櫻花APETALA1基因之選殖與序列分析

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