This study investigated the contents of antioxidants and antioxidant activities of leaf extract and its soluble fractions of Psychotria rubra. Results revealed that ethyl acetate soluble fraction had the highest contents of the total phenolic content (411.01 mg of GAE/g) and the total flavonoid content (210.56 mg of QE/g), among the examined leaf extract and its soluble fractions. In DPPH radical scavenging assay, leaf extract and ethyl acetate soluble fraction showed better DPPH radical scavenging activity. Ethyl acetate soluble fraction also exhibited the best reducing power and trolox equivalent antioxidant capacity. Based on the results, P. rubra leaf extract and ethyl acetate soluble fraction possess the superior antioxidant activity, which is worthy of further research and utilization.