The shoots of Alpinia speciosa K. Schum were induced successfully from the shoot tips cultured on MS medium containing BA 2 mg/L and NAA 0.05 mg/L. Each explant including two or three buds was suggested to subculture for multiplication. Every 1.5 months each explant proliferated 3 times when the medium containing BA 2 or 4 mg/L. The explants cultured in liquid grow bigger and faster than on solid medium.The NAA 4 mg/L treatment has the highest root number 7.34, with the root length of 0.76 cm that is suitable for transplant and all the offspring remain their variegated characters.