以手提式近紅外線分光光度計測定稻米蛋白質及直鏈澱粉含量

Determining the Amylose and Protein Content of Rough Rice by a Portable Near-Infrared Spectroscopy

李汪盛(Wang-Sheng Li) ； 蕭介宗(Jai-Tsung Shaw)

《桃園區農業改良場研究彙報》 39期 (1999/12) Pp. 28-40

https://doi.org/10.29567/ZHWHGX.199912.0002

摘要

利用化學分析方法分析稻米化學組成，可以用來評估稻米品質，但是費時、價格高且容易造成環境污染。本研究研發之手提式近紅外線分光光度計包括光纖探針、二極體陣列式光譜儀（範圍950－1650 nm）及手提電腦等設備，可應用於快速偵測整粒稻穀蛋白質及直鏈澱粉含量，樣本含水率為13%（w.b.），溫度為25℃。經由標準化轉換處理後之光譜，再利用部份最小平方法分析，分析結果顯示，利用9個因子之部份最小平方法所建立之整粒稻穀直鏈澱粉含量校正線，驗證樣本之判定係數（r^2）為0.88，驗證標準偏差（SEP）為0.52%。利用12個因子之部份最小平方法所建立之整粒稻穀蛋白質含量校正線，驗證樣本之判定係數為0.88，驗證標準偏差為0.28%。

並列摘要

Measuring the chemical compositions of rough rice by wet chemical methods can be used for evaluating rice quality, but they are time-consuming, costly, and polluting. A portable near-infrared spectroscopy system which includes a fiber optic probe, a diode array spectrophotometer, ranging from 950 nm to 1650 nm, together with a portable computer were developed to calibrate protein and amylose content of whole-kernel rough rice at 25℃ and 13% moisture content for a quick evaluation of rice quality. With standard normal variate pretreatment of each original absorbance spectrum and then analyses by partial least-squares regression, the calibration curve of amylose content by employing 9 principal-component PLSR model had r-squared of validation and SEP 0.88, 0.52 %, respectively. For protein content by employing 12 principal-component PLSR model, r-squared of validation and SEP were 0.88 and 0.28 %, respectively.

並列關鍵字

Portable near-infrared spectroscopy ； Rough rice ； Protein and amylose contents