黃根節蘭授粉後DNA微注射處理對莢果發育之影響

Capsule Development as Influenced by DNA Microinjection after Pollination in Calanthe sieboldii Decne. Ex Reg.

李淑真(Shu-Jen Lee) ； 廖芳心(Fang-Shin Liao) ； 鄭隨和(Shui-Ho Cheng)

《桃園區農業改良場研究彙報》 61期 (2007/06) Pp. 51-58

https://doi.org/10.29567/ZHWHGX.200706.0006

摘要

本試驗目的在釐定黃根節蘭（Calanthe sieboldii Decne. ex Reg.）DNA微注射處理轉移外來基因的最適時間，並調查授粉後天數DNA微注射處理外來基因對莢果發育之影響。螢光顯微鏡觀察黃根節蘭授粉後花粉管生長及受精情形，不同時間結果顯示，授粉後48小時花粉塊已萌芽，授粉後60天完成受精。於授粉後不同時間進行DNA微注射處理外來基因，結果以授粉後55天所得之莢果結實率達100%為最高，於授粉後60天所得之莢果鮮重為2.60g最重。

關鍵字

黃根節蘭 ； 授粉 ； 花粉管發芽 ； DNA微注射

並列摘要

This study is to investigate the optimum time for DNA microinjection via pollen tube pathway and effect of DNA microinjection on the capsule development at different duration after pollination in C. sieboldii Decne. The pollen was found germinated at 48 hours and fertilized at 60 days after pollination. The percentage in capsule set was 100% in the treatment of DNA microinjection at 55 days after pollination, but the highest fresh weight which was 2.6g per capsule was shown in the treatment microinjected at 60 days after pollination. These results indicated that the optimum time for DNA microinjection via pollen tube pathway was at 55 days after pollination.

並列關鍵字

Calanthe sieboldii Decne. ； pollination ； DNA microinjection ； pollen tube pathway