Eight qualitative and nine quantitative traits were investigated to estimate genetic variation of D. matsudae Hayata in Taiwan. Results of the distribution frequency showed that stem color and bud color were suitable for genetic variation assessment and species identification. Petiole d iameter, terminal leaf width and medial leaf width could be used as important indicators for species identification. Cluster analysis showed obviously overlapping distribution in regions. This study established useful information for germplasm collection, evaluation and application of D. matsudae Hayata in Taiwan