Diurnal Variation in Cell Population Kinetics of Normal Hamster Cheek Pouch and Hamsters with Squamous Cell Carcinoma

正常倉鼠頰囊袋與患類上皮癌袋中DNA合成期之表皮細胞及細胞叢集與一日間時辰節律變化之關係

林立民(Li-Min Lin) ； Robert A. Goepp

《The Kaohsiung Journal of Medical Sciences》 2卷12期 (1986/12) Pp. 745-753

https://doi.org/10.6452/KJMS.198612.0745

摘要

使用0.5%二笨甲葸(9,10-dimethyl-1,2 benzanthracene, DMBA)於倉鼠(Golden Syrian hamster)頰囊袋定期塗抹(每週三次)12週後於實驗組中，絕大多數之倉鼠均產生類上皮癌，在實驗組與對照組中於DNA合成其上癌細胞(含細胞叢集)及基底細胞(含細胞叢集)均呈周日節律性狀況(diurnal variation)前者之幅度及頻率高而密，後者則偏於低且平坦緩慢，被標幟的細胞及細胞叢集之百分率在統計學上有顯著之差異，前者約為後者之9.6倍，且於一天中之大部份時刻呈不隨意分佈，其間最高值與最低值有顯著差異。在進入DNA合成期之細胞與細胞叢數之百分率比癌前期周日變化報告之實驗組增加1.7倍，然而比對照組之數目減少2.7倍。正合乎前實驗之推論，在DNA合成期之癌細胞呈周日節律性分佈及部份呈細胞叢狀，證實了「動力同部」(symphokinesis)之可能性，動力同部就是一群細胞在進入DNA合成期或細胞分裂期時呈叢狀且和諧狀的不隨意分佈，它不僅在幼鼠口腔中之底層細胞被報告過。對於已確知對放射線敏感之癌細胞而論，本篇之發現強調了選擇時辰治療癌症之重要觀念。

並列摘要

After twelve weeks of regular topical appication of 0.5% 9,10-dimethyl-1, 2-benzanthracene (DMBA), a marked diurnal variation was found in the DNA synthesis of squamous cell carcinoma in hamster cheek pouches. Such rhythms were barely found in the control group.
A statistically significant difference occurred between the high values and low values of labeling and clustering indexes of the experimental group. The labelling and clustering indexes in the experimental group were 9.6 times greater than that of the control group. The experimental group also showed non-random distribution of labeled tumor cells.
The experimental group of this study showed approximately 1.7 times more cells in DNA synthesis than were found in the experimental groups of our previous study. The control group had approximately 2.7 times less labelled basal cells in DNA synthesis than that of the previous study. This tends to confirm that in the previous experiment the control group was indirectly affected by a painted experimental side. These findings prove that the longer DMBA was used, the more epithelial cells in clusters entered DNA synthesis.
The rhythmic pattern of tumor cell clustering provides evidence of “symphokinesis”. It is suggested that diurnal variation in malignant tumors may provide opportunities for improving treatment.

並列關鍵字

DMBA ； diurnal variation ； cluster