影響血小板凝集試驗諸因子及年齡關係之探討

Study on the Effect of Various Conditions and Age to Blood Platelet Aggregation Test

林獻誠(Hsien-Cheng Lin) ； 劉宏文(Hon-Wen Liu) ； 林勝豐(Sheng-Fung Lin)

《The Kaohsiung Journal of Medical Sciences》 6卷12期 (1990/12) Pp. 636-642

https://doi.org/10.6452/KJMS.199012.0636

摘要

為求得血小板凝集試驗之最佳條件，採血後於塑膠管內添加不同抗凝固劑，經過不同時間的靜置以及不同的溫度，跟加入不同濃度的adenosine diphosphate (ADP)，collagen及epinephrine試劑，和不同的血小板數目分別測其小板凝集率，並觀察各年齡層間之差異，所得最佳結果如下：
(一)以3.8%枸櫞酸為抗凝固劑。
(二)測定檢體應在收集後4小時內完成。
(三)適當溫度為25℃至37℃之間。
(四)血小板數目為2~4×105/μl。
(五)ADP，collagen，epinephrine 之適當最終濃度分別為5μg/ml及μM。
並觀察得知血小板凝集率會隨年齡增加而昇高。

並列摘要

The result of the platelet aggregation test was affected by various conditions. We performed the platelet aggregation test by changing different conditions, inlcuding:
1. adding different anticoagulants
2. interval between sampling and standing
3. platelet count
4. the concentrations of different reagents (ADP, Epinephrine and Collagen) respectively and the aggregation rate of blood platelet were compared among different age groups.
The best condition for platelet aggregation rate was obtained as follows: 1) Citric acid of 3.8% is the best anticoagulant. 2) Test should be completed within 4 hours upon sample collection. 3) The optimal incubation temperature is between 25° to 37℃. 4) Platelet count of 2 to 4 x l05/μl gives the most constant result. 5) The optimal concentration for working solution of ADP, collagen and Epinephrine are 5.0μm, 5.0 μg/ml and 50 μM respectively. 6) The aggregation rate of blood platelet increases with age.

並列關鍵字

platelet aggregation ； adenosine diphosphate ； epinephrine