Clonal Analysis in Acute Myeloid Leukemia by Polymerase Chain Reaction

利用聚合酶連鎖反應於急性骨髓性白血病之單源性分析

陳田柏(Tyen-Po Chen) ； 劉大智(Ta-Chih Liu) ； 張建國(Jan-Gowth Chang) ； 何金滿(Chin-Man Ho) ； 張肇松(Chao-Sung Chang) ； 黃叔牧(Shu-Mun Hwang) ； 林勝豐(Sheng-Fung Lin)

《The Kaohsiung Journal of Medical Sciences》 11卷4期 (1995/04) Pp. 191-196

https://doi.org/10.6452/KJMS.199504.0191

摘要

我們利幅合酶連鎖反應(PCR)來增幅X染色體上磷酸甘油酸激媒基因(PGK)上的一段多形性片段，同時合併利用甲基敏感性限制酶來認知具活性的X染色體，用於檢查台灣婦女中此基因異種接合的頻率並分析18個女性急性骨髓性白血病(AML)的單源性。
我們利用頭髮濾泡作為正常組給對照組，發現在102個血液學正常的女姓和18個女性病人中，PGK基因在台灣女性的異種接合發生頻率是35%(42/120)。在5個急性骨髓性白血病病人中，可發現當疾病初發性其白血病芽球表現X染色體不活化之單源性，而當病人緩解時則回復多源性之表現。在5人中有2人當疾病復發時，則又回復了單源性之表現。
我們也發現頭髮濾泡是很方便且不經侵襲性過程，容易取得之正常對照組織。

並列摘要

We have used PCR to amplify a polymorphic portion of the X-chromosome linked phosphoglycerate kinase gene (PGK) combined with a methylation-sensitive restriction enzyme digestion of the active x chromosome to examine the frequency of heterozygosity in Taiwanese females and analyze clonality in 18 female patients with acute myeloid leukemia (AML). We used hair follicles as normal tissue control. We found that the incidence of heterozygosity of the PGK gene in 102 hematological normal females and 18 patients tested was 35% (42/120). in five AML patients, a monoclonal X-inactivation pattern of leukemic blasts was found at presentation, which then returned to polyclonal at remission. In two of these five cases, a monoclonal pattern recurred at relapse. We also found that the hair follicles were readily accessible normal tissue for control, were easy to obtain, and were non-invasive.

並列關鍵字

phosphoglycerate kinase gene ； AML ； PCR