Comparison of the Genetic Variation in Type 1 Dengue Virus Isolates in Taiwan, 1987-1992

本研究以1987至1992年問於臺灣分離的第一型登革熱病毒8株（含2株境外移入株），萃取病毒RNA並進行反轉錄聚合醃連鎖反應(reverse transcriptase-polymerase chain reaction, RT-PCR)，再以基因選殖的方法定出各株的核苷酸序列（E/NS1 junction一段490bp基因）及肢基酸序列（164個肢基酸）。由核苷酸序列可知本土型株間的差異較小（0.61%至2.24%），而境外移入株與其它株間的差異較大（2.04%至4.29%）。我們可推論本土型登革熱病毒核苷酸序列具有地理相似性(geographic similarity)。另外本土株與原型株(prototype strain)在核苷酸序列的差異較一致(5.10%-5.92%)。而境外移入株與原型株間的差異由3.89%至7.14%。在胺基酸序列各株僅有1至2個胺基酸發生變化，乃因為核苷酸序列的變異都是點突變(point mutation)，且都發生在遺傳密碼的第三個核苷酸，而呈現寂靜變化(silent variation)。

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並列摘要

In this study, the genetic variation of eight dengue-1 strains isolated in Taiwan from 1987 to 1992 (including two imported strains) were studied. A fragment of 490 nucleotides from the E/NS1 junction was amplified and studied by reverse transcriptionpolymerase chain reaction (RT-PCR) and vector cloning. By comparing the nucleotide sequences of these strains, it was found that local strains in Taiwan shared geographic similarity. The nucleotide differences among local strains were less (0.61% to 2.24%) than the differences between imported strains and other strains (2.04% to 4.29%). The differences of nucleotide sequences between local strains and the prototype remain constant (5.10%-5.29%). However, the differences in nucleotide sequences between imported strains and the prototype ranged from 3.89% to 7.14%. Since the point mutations occurred in the wobble position of the codon, only conserved changes resulted in the amino acid among the isolates.

並列關鍵字

dengue-1 strain ； reverse transcription-polymerase chain reaction

國際替代計量

Comparison of the Genetic Variation in Type 1 Dengue Virus Isolates in Taiwan, 1987-1992

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